Immobilization of α-amylase onto cyclic carbonate bearing hybrid material

被引：51

作者：

Turunc, Oguz ^{[1
]}

Kahraman, Memet Vezir ^{[1
]}

Akdemir, Zuemruet Seden ^{[1
]}

Kayaman-Apohan, Nilhan ^{[1
]}

Gungor, Atilla ^{[1
]}

机构：

[1] Marmara Univ, Fac Arts & Sci, Dept Chem, TR-34722 Goztepe, Turkey

来源：

FOOD CHEMISTRY | 2009年 / 112卷 / 04期

关键词：

alpha-Amylase; Enzyme immobilization; Cyclic carbonate; Sol gel; UV curing; COVALENT IMMOBILIZATION; ALKALINE-PHOSPHATASE; VINYLENE CARBONATE; ENZYME-ACTIVITY; STABILITY; HYDROLYSIS; BEADS; IMPROVEMENT; CARRIERS; RESIN;

D O I：

10.1016/j.foodchem.2008.07.024

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Under ambient conditions, 4-((3-trimethoxysilyl)propoxy)methyl)1,3-dioxolan-2-one was synthesized from 3-glycidyloxypropyl trimethoxysilane. Methacrylate and cyclic carbonate functional hybrid matrix was prepared by sol-gel method. alpha-Amylase was covalently bounded onto the matrix via cyclic carbonate functionality. Immobilization yield was found as 34.4 +/- 2.6 mg per gram of hybrid matrix. The maximum activity was observed at pH 6.5. Immobilization did not change the pH-dependency of the enzyme. The immobilized enzyme had a higher activity at elevated temperature (50-80 degrees C) than the free one. Immobilized enzyme exhibited 80% activity after 20 runs and 69% after 30 runs. Free enzyme lost its activity completely within 15 days. Immobilized enzyme lost only 30% of its activity in 25 days. V-max values for the free and immobilized enzymes were calculated as 58 x 10(-3) and 5.2 x 10(-3) mg/ml min(-1), respectively. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：992 / 997

页数：6

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