Immobilization of α-amylase onto cyclic carbonate bearing hybrid material

被引:51
作者
Turunc, Oguz [1 ]
Kahraman, Memet Vezir [1 ]
Akdemir, Zuemruet Seden [1 ]
Kayaman-Apohan, Nilhan [1 ]
Gungor, Atilla [1 ]
机构
[1] Marmara Univ, Fac Arts & Sci, Dept Chem, TR-34722 Goztepe, Turkey
关键词
alpha-Amylase; Enzyme immobilization; Cyclic carbonate; Sol gel; UV curing; COVALENT IMMOBILIZATION; ALKALINE-PHOSPHATASE; VINYLENE CARBONATE; ENZYME-ACTIVITY; STABILITY; HYDROLYSIS; BEADS; IMPROVEMENT; CARRIERS; RESIN;
D O I
10.1016/j.foodchem.2008.07.024
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Under ambient conditions, 4-((3-trimethoxysilyl)propoxy)methyl)1,3-dioxolan-2-one was synthesized from 3-glycidyloxypropyl trimethoxysilane. Methacrylate and cyclic carbonate functional hybrid matrix was prepared by sol-gel method. alpha-Amylase was covalently bounded onto the matrix via cyclic carbonate functionality. Immobilization yield was found as 34.4 +/- 2.6 mg per gram of hybrid matrix. The maximum activity was observed at pH 6.5. Immobilization did not change the pH-dependency of the enzyme. The immobilized enzyme had a higher activity at elevated temperature (50-80 degrees C) than the free one. Immobilized enzyme exhibited 80% activity after 20 runs and 69% after 30 runs. Free enzyme lost its activity completely within 15 days. Immobilized enzyme lost only 30% of its activity in 25 days. V-max values for the free and immobilized enzymes were calculated as 58 x 10(-3) and 5.2 x 10(-3) mg/ml min(-1), respectively. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:992 / 997
页数:6
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