Fifteen polymorphic microsatellite loci in Wiebesia pumilae (Hill) (Agaonidae)

被引：7

作者：

Liu, Min ^{[1
]}

Wang, Xiao-Yan ^{[1
]}

Cui, Min-Yan ^{[1
]}

Chen, Yan ^{[1
,2
]}

机构：

[1] E China Normal Univ, Dept Environm Sci, Shanghai 200062, Peoples R China

[2] Mianyang Normal Univ, Key Lab Mol Biol & Biopharmaceut, Mianyang 621000, Sichuan, Peoples R China

来源：

CONSERVATION GENETICS RESOURCES | 2009年 / 1卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Wiebesia pumilae; Microsatellite loci; Genetic diversity; Mutualism;

D O I：

10.1007/s12686-009-9046-3

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Pollinating fig wasps and their hosts establish a paradise for coevolution and sex ratio studies. High polymorphic molecular markers would be a great assistance for such studies. We developed 15 microsatellite markers from Wiebesia pumilae, the obligate pollinator of Ficus pumila, and estimated the polymorphism with 22 individual wasps from Yongjia County, Zhejiang of China. A total of 74 alleles were detected in the 15 loci and the alleles per locus ranged from 2 to 9. The observed (HO) and expected (HE) heterozygosities were 0.190-0.727 and 0.483-0.831, respectively. Significant deviation from Hardy-Weinberg equilibrium was found at seven loci due to heterozygote deficits. No locus pair showed significant linkage disequilibrium. These microsatellite markers will provide a useful tool for studying the genetic structure of W. pumilae, and its coevolution with the host F. pumila.

引用

页码：189 / 191

页数：3

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