Identification of novel retromer complexes in the mouse testis

被引：15

作者：

Kim, Ekyune ^{[1
]}

Lee, Jae-Woong ^{[1
]}

Baek, Dong-Chul ^{[1
,2
]}

Lee, Sang-Rae ^{[1
]}

Kim, Myeong-Su ^{[1
]}

Kim, Sang-Hyun ^{[1
]}

Imakawa, Kazuhiko ^{[3
]}

Chang, Kyu-Tae ^{[1
]}

机构：

[1] Korea Res Inst Biosci & Biotechnol, Natl Primate Res Center, Ochang Eup 363883, Chung Buk, South Korea

[2] Jeju Natl Univ, Fac Biotechnol, Jejusi 690756, Jeju, South Korea

[3] Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 375卷 / 01期

关键词：

retromer complex; vacuolar protein sorting; embryonic development; endosome; spermatogenesis;

D O I：

10.1016/j.bbrc.2008.07.067

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A family of vacuolar protein sorting (Vps) proteins, which are components of mammalian retromer complex, has been studied in the mouse. Vps26a is known as a retromer component that plays an important role in embryonic development: however, its cell-type expression and precise role remain to be elucidated. In this study, we identified a new isoform of Vps26a, called Vps26aT, which was expressed specifically in the mouse testis. Diverse expression patterns of Vps26 variants in mouse tissues were determined by Western blot and RT-PCR analyses, and the direct interaction of Vps26aT with Vps35 was also demonstrated by immunoprecipitation and pull-down assay Using antibodies raised against each Vps component. Our results revealed that the retromer complex Could be formed from different Vps26 isoforms in a tissue-specific manner, resulting in more than two types of the retromer complex, including the Vps26a-Vps29-Vps35, Vps26aT-Vps29-Vps35, and Vps26b-Vps29-Vps35 complexes in mouse tissues. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：16 / 21

页数：6

共 20 条

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