Impact of silicon-based quantum dots on the antioxidative system in white muscle of Carassius auratus gibelio

被引:8
作者
Stanca, Loredana [1 ]
Petrache, Sorina Nicoleta [1 ]
Radu, Mihaela [1 ]
Serban, Andreea Iren [1 ,2 ]
Munteanu, Maria Cristina [1 ]
Teodorescu, Daniela [1 ]
Staicu, Andreea Cristina [1 ]
Sima, Cornelia [3 ]
Costache, Marieta [1 ]
Grigoriu, Constantin [3 ]
Zarnescu, Otilia [1 ]
Dinischiotu, Anca [1 ]
机构
[1] Univ Bucharest, Fac Biol, Dept Biochem & Mol Biol, Bucharest 050095, Romania
[2] Univ Agr Sci & Vet Med, Dept Preclin Sci, Fac Vet Med, Bucharest 050097, Romania
[3] Natl Inst Laser Plasma & Radiat Phys, Laser Dept, Bucharest 077125, Romania
关键词
Fish white muscle; Silicon-based quantum dots; Malondialdehyde; Reduced glutathione; Antioxidative enzymes; Protein oxidation; OXIDATION PROTEIN PRODUCTS; TROUT ONCORHYNCHUS-MYKISS; SEMICONDUCTOR NANOCRYSTALS; TISSUE DISTRIBUTION; NANOPARTICLES; STRESS; FISH; ZEBRAFISH; TOXICITY; CELL;
D O I
10.1007/s10695-011-9582-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Silicon-based quantum dots were intraperitoneally injected in individuals of Carassius auratus gibelio. Their effects on white muscle were investigated by following their distribution and impact on the antioxidative system. The GSH level significantly increased after 1 and 3 days of exposure by, respectively, 85.3 and 25.4%. Seven days later, GSH levels were similar to control concentrations. MDA concentration rose after three days by 46.9% and remained at the same level after 7 days. Protein thiol levels significantly decreased by 6.7 and 8.1% after 3 and 7 days, whereas advanced oxidation protein products increased by 12.7, respectively, 28.1% in the same time intervals. The protein reactive carbonyl groups were raised only after the first day of exposure and returned to the control level later on. SOD specific activity increased up to 48% after 7 days, while CAT activity increased by 328, 176, and 26% after 1, 3, and 7 days of treatment. GST specific activity was up-regulated by 87, 18, and 9%, while GR activity increased by 68, 34, and 9%. G6PD activity was up-regulated by 12, 22, and 50%, whereas GPx activity raised by 75 and 109% compared to control after, respectively, 1, 3, and 7 days. Our results suggest that oxidative stress induced by silicon-based quantum dots was not strong enough to cause permanent damage in the white muscle of crucian carp.
引用
收藏
页码:963 / 975
页数:13
相关论文
共 90 条
[1]   Protein oxidation in the brain in Alzheimer's disease [J].
Aksenov, MY ;
Aksenova, MV ;
Butterfield, DA ;
Geddes, JW ;
Markesbery, WR .
NEUROSCIENCE, 2001, 103 (02) :373-383
[2]   Evolutionary mutant models for human disease [J].
Albertson, R. Craig ;
Cresko, William ;
Detrich, H. William, III ;
Postlethwait, John H. .
TRENDS IN GENETICS, 2009, 25 (02) :74-81
[3]   The role of advanced oxidation protein products in regulation of dendritic cell function [J].
Alderman, CJJ ;
Shah, S ;
Foreman, JC ;
Chain, BM ;
Katz, DR .
FREE RADICAL BIOLOGY AND MEDICINE, 2002, 32 (05) :377-385
[4]   Type II skeletal myofibers possess unique properties that potentiate mitochondrial H2O2 generation [J].
Anderson, EJ ;
Neufer, PD .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2006, 290 (03) :C844-C851
[5]  
[Anonymous], 1974, Catalase. Methods Enzym. Anal., DOI DOI 10.1016/B978-0-12-091302-2.50032-3
[6]   Quantum dot anti-CD conjugates: Are they potential photosensitizers or potentiators of classical photosensitizing agents in photodynamic therapy of cancer? [J].
Bakalova, R ;
Ohba, H ;
Zhelev, Z ;
Nagase, T ;
Jose, R ;
Ishikawa, M ;
Baba, Y .
NANO LETTERS, 2004, 4 (09) :1567-1573
[7]   Quantum Dots for In Vivo Small-Animal Imaging [J].
Bentolila, Laurent A. ;
Ebenstein, Yuval ;
Weiss, Shimon .
JOURNAL OF NUCLEAR MEDICINE, 2009, 50 (04) :493-496
[8]  
Bergmeyer HU., 1983, METHODS ENZYMATIC AN, VVI., P258
[9]  
Beutler E., 1984, RED CELL METABOLISM, P68
[10]   Semiconductor nanocrystals as fluorescent biological labels [J].
Bruchez, M ;
Moronne, M ;
Gin, P ;
Weiss, S ;
Alivisatos, AP .
SCIENCE, 1998, 281 (5385) :2013-2016