The influence of the size and aspect ratio of anisotropic, porous CaCO3 particles on their uptake by cells

被引:135
作者
Parakhonskiy, Bogdan [1 ,2 ]
Zyuzin, Mikhail V. [3 ]
Yashchenok, Alexey [2 ,4 ]
Carregal-Romero, Susana [3 ]
Rejman, Joanna [3 ]
Moehwald, Helmuth [4 ]
Parak, Wolfgang J. [3 ]
Skirtach, Andre G. [4 ,5 ,6 ]
机构
[1] Russian Acad Sci, Shubnikov Inst Crystallog, Moscow, Russia
[2] Saratov NG Chernyshevskii State Univ, Inst Nanostruct & Biosyst, Saratov, Russia
[3] Univ Marburg, Fachbereich Phys, D-35032 Marburg, Germany
[4] Max Planck Inst Colloids & Interfaces, Dept Interfaces, Potsdam, Germany
[5] Univ Ghent, NanoBiophoton, B-9000 Ghent, Belgium
[6] Univ Ghent, Dept Mol Biotechnol, B-9000 Ghent, Belgium
来源
JOURNAL OF NANOBIOTECHNOLOGY | 2015年 / 13卷
关键词
Calcium carbonate; Anisotropic; Uptake; Cells; Internalisation; POLYELECTROLYTE MULTILAYER CAPSULES; CALCIUM-CARBONATE MICROPARTICLES; MESENCHYMAL STEM-CELLS; DRUG-DELIVERY; CELLULAR UPTAKE; GOLD NANOPARTICLES; MAMMALIAN-CELLS; CANCER-CELLS; INTERNALIZATION PATHWAYS; SILVER NANOPARTICLES;
D O I
10.1186/s12951-015-0111-7
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: Recent reports highlighting the role of particle geometry have suggested that anisotropy can affect the rate and the pathway of particle uptake by cells. Therefore, we investigate the internalization by cells of porous calcium carbonate particles with different shapes and anisotropies. Results: We report here on a new method of the synthesis of polyelectrolyte coated calcium carbonate particles whose geometry was controlled by varying the mixing speed and time, pH value of the reaction solution, and ratio of the interacting salts used for particle formation. Uptake of spherical, cuboidal, ellipsoidal (with two different sizes) polyelectrolyte coated calcium carbonate particles was studied in cervical carcinoma cells. Quantitative data were obtained from the analysis of confocal laser scanning microscopy images. Conclusions: Our results indicate that the number of internalized calcium carbonate particles depends on the aspect ratio of the particle, whereby elongated particles (higher aspect ratio) are internalized with a higher frequency than more spherical particles (lower aspect ratio). The total volume of internalized particles scales with the volume of the individual particles, in case equal amount of particles were added per cell.
引用
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页数:13
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