Separation and identification of corticosterone metabolites by liquid chromatography electrospray ionization mass spectrometry

被引：24

作者：

Miksík, I

Vylitová, M

Pácha, J

Deyl, Z

机构：

[1] Acad Sci Czech Republ, Inst Physiol, CZ-14220 Prague 4, Czech Republic

[2] Charles Univ, Sch Med 2, Dept Physiol, CZ-15000 Prague, Czech Republic

来源：

JOURNAL OF CHROMATOGRAPHY B | 1999年 / 726卷 / 1-2期

关键词：

corticosterone;

D O I：

10.1016/S0378-4347(99)00028-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-performance liquid chromatography coupled to atmospheric pressure ionization-electrospray ionization mass spectrometry (API-ESI-MS) was investigated for the analysis of corticosterone metabolites; their characterization was obtained by combining the separation on Zorbax Eclipse XDB C-18 column (eluted with a methanol-water-acetic acid gradient) with identification using positive ion mode API-ESI-MS and selected ion analysis. The applicability of this method was verified by monitoring the activity of steroid converting enzymes (20 beta-hydroxysteroid dehydrogenase and 11 beta-hydroxysteroid dehydrogenase) in avian intestines. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：59 / 69

页数：11

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