Comparative Gene Expression Profiling in Human Cumulus Cells according to Ovarian Gonadotropin Treatments

被引：32
作者：
Assou, Said [1 ,2 ]
Haouzi, Delphine [2 ]
Dechaud, Herve [1 ,2 ,3 ]
Gala, Anna [1 ,2 ,3 ]
Ferrieres, Alice [1 ,2 ,3 ]
Hamamah, Samir [1 ,2 ,3 ]
机构：
[1] Univ Montpellier I, UFR Med, Montpellier, France
[2] Hop Saint Eloi, CHU Montpellier, INSERM, Inst Res Biotherapy,U1040, F-34295 Montpellier, France
[3] Hop Arnaud Villeneuve, CHU Montpellier, ART PGD Dept, F-34295 Montpellier, France
来源：
BIOMED RESEARCH INTERNATIONAL | 2013年 / 2013卷
关键词：
HUMAN GRANULOSA-CELLS; HIGHLY PURIFIED HMG; RECOMBINANT FSH; GROWTH-FACTOR; EMBRYO DEVELOPMENT; OOCYTE COMPLEXES; NEUROPEPTIDE-Y; STIMULATION; FOLLICLE; CULTURE;
D O I：
10.1155/2013/354582
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In in vitro fertilization cycles, both HP-hMG and rFSH gonadotropin treatments are widely used to control human follicle development. The objectives of this study are (i) to characterize and compare gene expression profiles in cumulus cells (CCs) of periovulatory follicles obtained from patients stimulated with HP-hMG or rFSH in a GnRH antagonist cycle and (ii) to examine their relationship with in vitro embryo development, using Human Genome U133 Plus 2.0 microarrays. Genes that were upregulated in HP-hMG-treated CCs are involved in lipid metabolism (GM2A) and cell-to-cell interactions (GJA5). Conversely, genes upregulated in rFSH-treated CCs are implicated in cell assembly and organization (COL1A1 and COL3A1). Interestingly, some genes specific to each gonadotropin treatment (NPY1R and GM2A for HP-hMG; GREM1 and OSBPL6 for rFSH) were associated with day 3 embryo quality and blastocyst grade at day 5, while others (STC2 and PTX3) were related to in vitro embryo quality in both gonadotropin treatments. These genes may prove valuable as biomarkers of in vitro embryo quality.
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页数：13
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