Chemical fragmentation for massively parallel sequencing library preparation

被引：3
作者：
Gyarmati, P. [1 ]
Song, Y. [1 ]
Hallman, J. [1 ]
Kaller, M. [1 ]
机构：
[1] KTH Royal Inst Technol, Sci Life Lab, Sch Biotechnol, Div Gene Technol, SE-17165 Solna, Sweden
来源：
JOURNAL OF BIOTECHNOLOGY | 2013年 / 168卷 / 01期
基金：
瑞典研究理事会;
关键词：
Fragmentation; High-throughput sequencing; Library preparation; DNA-DAMAGE; BIAS;
D O I：
10.1016/j.jbiotec.2013.08.020
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Fragmentation is essential in most library preparation protocols for use with massively parallel sequencing systems. Complexes that generate hydroxyl radicals, such as iron-EDTA, can be used to introduce random DNA cleavage. Here we describe a chemical fragmentation method that can be incorporated into library preparation protocols for next-generation sequencing workflows. This protocol has been validated by whole genome, amplicon and exome sequencing. Chemical fragmentation is a cost-effective alternative to current fragmentation methods that has no observable sequence bias and requires no instrumentation. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：95 / 100
页数：6
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