Characterization of avian strains of Pasteurella multocida by restriction endonuclease and amplified fragment length polymorphism

被引：6
|
作者：
Shivachandra, SB [1 ]
Kumar, AA [1 ]
Gautam, R [1 ]
Joseph, S [1 ]
Saxena, MK [1 ]
Chaudhuri, P [1 ]
Srivastava, SK [1 ]
机构：
[1] Catholic Univ Amer, Div Bacteriol & Mycol, Washington, DC 20064 USA
来源：
RESEARCH IN VETERINARY SCIENCE | 2006年 / 81卷 / 01期
关键词：
avian; AFLP; Pasteurella multocida; REA;
D O I：
10.1016/j.rvsc.2005.10.009
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Avian strains of Pasteurella multocida were typed by employing restriction endonuclease analysis (REA) and single enzyme-amplified fragment length polymorphism (AFLP) to evaluate their applicability for epidemiological studies of fowl cholera outbreaks. A total of 72 strains isolated from different avian species (chicken, duck, turkey, quail and goose) belonging to various geographical regions of India were characterized. REA using two different enzymes HhaI and HpaII produced 9 and 18 clusters respectively, whereas Single enzyme-AFLP recognized 32 patterns out of 72 strains typed. The study indicated that REA using HpaII is a simple and resource efficient method, however, further typing with more stringent and rapid method like Single enzyme-AFLP, could drastically enhance investigation in epidemiological studies of fowl cholera outbreaks. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码：8 / 18
页数：11
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