Ring-opening polymerization of ε-caprolactone catalyzed by a novel thermophilic esterase from the archaeon Archaeoglobus fulgidus

被引：45

作者：

Ma, Jiutong ^{[1
]}

Li, Quanshun ^{[1
]}

Song, Bo ^{[1
]}

Liu, Donglai ^{[1
]}

Zheng, Baisong ^{[1
]}

Zhang, Zuoming ^{[1
]}

Feng, Yan ^{[1
]}

机构：

[1] Jilin Univ, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130023, Peoples R China

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2009年 / 56卷 / 2-3期

关键词：

Thermophilic esterase; epsilon-Caprolactone; Ring-opening polymerization; LIPASE; LACTONES; POLYESTERS;

D O I：

10.1016/j.molcatb.2008.03.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ring-opening polymerization of epsilon-caprolactone catalyzed by a novel thermophilic esterase from the archaeon Archaeoglobus fulgidus was successfully conducted in organic solvents. The effects of enzyme concentration, temperature, reaction time, reaction medium. and water activity on monomer conversion and product molecular weight were investigated. Poly(epsilon-caprolactone) was obtained in almost 100% of the monomer conversion, with a number-average molecular weight of 1400 in toluene at 80 degrees C for 72 h. Furthermore, the Michaelis-Menten kinetic analysis showed that the enzyme had the highest affinity for epsilon-caprolactone, with a K-m value of 0.093 mol/l compared with other reported lipases. The possible structural and energetic effects of the enzyme on the K-m value were investigated, using molecular docking studies. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：151 / 157

页数：7

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