Study of long-term viability of endothelial cells for lab-on-a-chip devices

被引：17

作者：

Voiculescu, Ioana ^{[1
]}

Li, Fang ^{[2
]}

Liu, Fei ^{[1
]}

Zhang, Xudong ^{[1
]}

Cancel, Limary M. ^{[3
]}

Tarbell, John M. ^{[3
]}

Khademhosseini, Ali ^{[4
,5
,6
,7
]}

机构：

[1] CUNY City Coll, Dept Mech Engn, New York, NY 10031 USA

[2] Intelligent Automat Inc, Rockville, MD 20855 USA

[3] CUNY City Coll, Dept Biomed Engn, New York, NY 10031 USA

[4] Harvard Univ, Sch Med, Brigham & Womens Hosp, Ctr Biomed Engn,Dept Med, Cambridge, MA 02139 USA

[5] MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA

[6] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

[7] Tohoku Univ, World Premier Int Adv Inst Mat Res WPI AIMR, Sendai, Miyagi 9808577, Japan

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2013年 / 182卷

基金：

美国国家科学基金会;

关键词：

Long-term cell viability; ECIS; Lab-on-a-chip; IMPEDANCE SPECTROSCOPY; NEURONAL NETWORKS; BIOSENSOR SYSTEM; MAMMALIAN-CELLS; TISSUE-CULTURE; REAL-TIME; MICROMOTION; PERFUSION; DESIGN; MOTION;

D O I：

10.1016/j.snb.2013.03.030

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Biosensors that employ live mammalian cells as sensing elements require precise information about the cell longevity. These biosensors could be stored in an incubator and used during the lifetime of the cells. This paper is a study of the longevity of bovine aortic endothelial cells (BAECs) that are used as sensorial component for cell-based biosensors. Different types of polydimethylsiloxane (PDMS) cell culturing chambers along with the culturing conditions required for BAECs to survive long term in lab on a chip systems are presented. The electric cell-substrate impedance (ECIS) technique was used to monitor cell viability over extended time periods. Media was automatically recirculated over the cells by a portable pump, in order to create the conditions required for testing the sensor in the field. It was demonstrated the BAECs could survive up to 37 days. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：696 / 705

页数：10

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