Determination of isoflavones in rat serum using liquid chromatography-tandem mass spectrometry with a highly efficient core-shell column

被引：9

作者：

Gavina, Jennilee M. A. ^{[1
]}

Priem, Jessica ^{[1
]}

Wood, Carla M. ^{[2
]}

Xiao, Chao Wu ^{[2
]}

Feng, Yong-Lai ^{[1
]}

机构：

[1] Hlth Canada, Exposure & Biomonitoring Div, Healthy Environm & Consumer Safety Branch, EHC, Ottawa, ON K1A 0K9, Canada

[2] Hlth Canada, Nutr Res Div, Hlth Prod & Food Branch, Ottawa, ON K1A 0K9, Canada

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2013年 / 405卷 / 08期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Isoflavones; Liquid chromatography-tandem mass spectrometry (HPLC-MS/MS); Core-shell column; Dual enzyme hydrolysis; Serum; LC-MS/MS METHOD; SOY ISOFLAVONES; PHYTOESTROGENS; QUANTIFICATION; IDENTIFICATION; METABOLITES; GENISTEIN; FLAVONOIDS; DAIDZEIN; PROTEIN;

D O I：

10.1007/s00216-012-6688-x

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Consumption and nutritional supplementation of soy and soy-based products have been linked to health benefits such as lower cholesterol and triglyceride levels, and decreased incidence of cardiovascular disease and diabetes. In this study, we have developed a sensitive, specific, and robust method using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for determination of serum isoflavones. A new highly efficient pentafluorophenyl phase core-shell column was first used to separate all isoflavones within 3 min, a separation time which is comparable to ultra-pressure liquid chromatography (UPLC) and micro-HPLC. A two-enzyme hydrolysis system with sulfatase and beta-glucuronidase has also been developed to improve the efficiency of deconjugation of conjugated isoflavones in serum. The corresponding conjugated isoflavones were used to evaluate recoveries. In addition to duplicates, the method of standard addition was also applied in sample analysis for quality control. The developed method was applied to the analysis of 32 serum samples and was shown to be specific, sensitive and reproducible.

引用

页码：2643 / 2651

页数：9

共 31 条

[1] Identification of phytoestrogens in bovine milk using liquid chromatography/electrospray tandem mass spectrometry [J].