Potential Application of Titanium Dioxide Nanoparticles in the Prevention of Osteosarcoma and Chondrosarcoma Recurrence

被引:38
作者
Sha, Baoyong [1 ,2 ]
Gao, Wei [1 ]
Han, Yulong [1 ,2 ]
Wang, ShuQi [3 ]
Wu, Jinhui [4 ]
Xu, Feng [1 ,2 ]
Lu, TianJian [2 ]
机构
[1] Xi An Jiao Tong Univ, Sch Life Sci & Technol, Minist Educ, Key Lab Biomed Informat Engn, Xian 710049, Peoples R China
[2] Xi An Jiao Tong Univ, Biomed Engn & Biomech Ctr, Xian 710049, Peoples R China
[3] Harvard Univ, Brigham & Womens Hosp, Dept Med, HST Ctr Biomed Engn,Med Sch, Cambridge, MA 02139 USA
[4] Nanjing Univ, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Jiangsu, Peoples R China
基金
中国国家自然科学基金; 高等学校博士学科点专项科研基金;
关键词
Titanium Dioxide (TiO2) Nanoparticles; Osteosarcoma; Chondrosarcoma; TOXICITY; GROWTH;
D O I
10.1166/jnn.2013.6081
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Osteosarcoma and chondrosarcoma are malignant bone tumors, and they significantly affect the life quality of patients including children and adults. The main treatment method is surgical amputation of the malignant lesion, despite that recurrence often occurs. Recently, it has been observed that TiO2 NPs killed HeLa cells effectively via photocatalysis in vitro, which indicates titanium dioxide (TiO2) nanoparticles (NPs) might be used to reduce the recurrence of osteosarcoma and chondrosarcoma by inducing cytotoxicity to bone tumor cells. In this study, we investigated the potential effects of TiO2 NPs in two cancer cell lines in vitro: U-2 OS (osteosarcoma) and SW 1353 (chondrosarcoma). We assessed cell viability, the levels of reactive oxygen species (ROS) and glutathione (GSH) after exposure to TiO2 NPs at different concentrations (0.1-100 mu g/ml) for varying exposure periods (12-48 hours). Compared to the NP-free control, TiO2 NPs induced cell death in a dosage-dependent and time-dependent manner. The median inhibitory concentration (IC50) of TiO2 NPs at 24 hours was 211.3 +/- 15.2 mu g/ml and 5408.8 +/- 45.9 mu g/ml for SW 1353 and U-2 OS cell lines, respectively. TiO2 NPs concentrations above 1 mu g/ml were more efficient to reduce the cell viability of SW 1353 than U-2 OS of NPs at all exposure times. The increased ROS and reduced GSH levels indicated that TiO2 NPs killed cancer cells through oxidative stress. These results suggested that the TiO2 NPs can be potentially used to minimize/prevent the recurrence of osteosarcoma and chondrosarcoma.
引用
收藏
页码:1208 / 1211
页数:4
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