A Highly Entangled Polymeric Nanoconstruct Assembled by siRNA and its Reduction-Triggered siRNA Release for Gene Silencing

被引：29

作者：

Namgung, Ran ^{[1
]}

Kim, Won Jong ^{[1
]}

机构：

[1] Pohang Univ Sci & Technol, Polymer Res Inst, BK Sch Mol Sci, Dept Chem, Pohang 790784, South Korea

来源：

SMALL | 2012年 / 8卷 / 20期

基金：

新加坡国家研究基金会;

关键词：

composite materials; nanostructures; self-assembly; small interfering RNA; gene silencing; bio-nanotechnology; RNA INTERFERENCE; DELIVERY CARRIERS; NANOGELS; CELLS; BIODEGRADATION; COMPARTMENTS; CONJUGATION; PH;

D O I：

10.1002/smll.201200496

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A nanoconstruct (NC) is developed from a biocompatible natural polymer and siRNA conjugates to deliver small interfering RNA (siRNA) target-specifically without cationic condensation reagents. This study reports a novel siRNA-mediated cross-linked NC produced by hybridizing two complementary single-stranded siRNAs that are conjugated to the polymer dextran via a disulfide linkage. The reducible disulfide bond between the siRNA and polymer allow siRNA release from the NC in the reducible cytoplasmic region after the NC enters the cell. In addition, when the NC contains the prostate-carcinoma-binding peptide aptamer DUP-1, it can selectively deliver siRNA into prostate cancer cells of the PC-3 lines; thus, the newly formulated NC has reduced the cytotoxicity and improved the efficacy of target-specific siRNA delivery. Moreover, this new concept of NCs using biocompatible siRNA and a neutral polymer may provide insightful knowledge for future directions for designing NCs for stimuli-responsive and advanced target-specific siRNA delivery.

引用

收藏

页码：3209 / 3219

页数：11

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