Evaluation of the Asparagine Synthetase Level in Leukemia Cells by Monoclonal Antibodies

被引：3

作者：

Kusano-Arai, Osamu ^{[4
]}

Iwanari, Hiroko

Mochizuki, Yasuhiro

Nakata, Hiromi

Kodama, Tatsuhiko ^{[2
]}

Kitoh, Toshiyuki ^{[3
]}

Hamakubo, Takao ^{[1
]}

机构：

[1] Univ Tokyo, Res Ctr Adv Sci & Technol, Dept Mol Biol & Med, Meguro Ku, Tokyo 1538904, Japan

[2] Univ Tokyo, Res Ctr Adv Sci & Technol, Lab Syst Biol & Med, Tokyo 1538904, Japan

[3] Aichi Med Univ, Dept Pediat, Nagakute, Aichi 48011, Japan

[4] Inst Immunol Co Ltd, Bunkyo Ku, Tokyo, Japan

来源：

HYBRIDOMA | 2012年 / 31卷 / 05期

关键词：

ACUTE LYMPHOBLASTIC-LEUKEMIA; EXPRESSION LEVELS; CYCLE ARREST; RESISTANCE; SENSITIVITY; BACULOVIRUS; PROTEINS; CANCER; LINES;

D O I：

10.1089/hyb.2012.0014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

L-Asparaginase (ASNase) is important for the treatment of childhood acute lymphoblastic leukemia. ASNase sensitivity has been shown to correlate with the asparagine synthetase (ASNS) protein content in acute lymphoblastic leukemia cell lines. However, there have been few studies to determine ASNS protein levels in human leukemias, since no appropriate monoclonal antibody is available for such quantitative analysis. In this study, we report the generation of anti-ASNS monoclonal antibodies, which are applicable to flow cytometry and enzyme-linked immunosorbent assay. These monoclonal antibodies should provide a valuable tool for the quantification of ASNS protein level and estimation of ASNase-resistance in leukemia cells.

引用

页码：325 / 332

页数：8

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