Cloning of Acyl-ACP Thioesterase FatA from Arachis hypogaea L. and Its Expression in Escherichia coli

被引：13

作者：

Chen, Gao ^{[1
,2
,4
]}

Peng, Zhen-ying ^{[1
,2
]}

Shan, Lei ^{[1
,2
]}

Xuan, Ning ^{[1
,2
]}

Tang, Gui-ying ^{[1
,2
]}

Zhang, Yan ^{[1
,2
]}

Li, Lan ^{[1
,2
,4
]}

He, Qing-fang ^{[1
,2
,3
]}

Bi, Yu-ping ^{[1
,2
,4
]}

机构：

[1] Shandong Acad Agr Sci, High Tech Res Ctr, Jinan 250100, Peoples R China

[2] Shandong Prov Key Lab Genet Improvement, Jinan 250100, Peoples R China

[3] Univ Arkansas, Dept Appl Sci, Little Rock, AR 72204 USA

[4] Shandong Normal Univ, Coll Life Sci, Key Lab Plant Stress, Jinan 250014, Peoples R China

来源：

JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | 2012年

基金：

中国国家自然科学基金; 美国国家科学基金会; 对外科技合作项目（国际科技项目）;

关键词：

CARRIER PROTEIN THIOESTERASE; DEVELOPING SEEDS; ACID SYNTHESIS; PURIFICATION; SPECIFICITY; GENES;

D O I：

10.1155/2012/652579

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, a full-length cDNA of the acyl-ACP thioesterase, AhFatA, was cloned from developing seeds of Arachis hypogaea L. by 3'-RACE. Sequence analysis showed that the open reading frame encodes a peptide of 372 amino acids and has 50-70% identity with FatA from other plants. Real-time quantitative PCR analysis revealed that AhFatA was expressed in all tissues of A. hypogaea L., but most strongly in the immature seeds harvested at 60 days after pegging. Heterologous expression of AhFatA in Escherichia coli affected bacterial growth and changed the fatty acid profiles of the membrane lipid, resulting in directed accumulation towards palmitoleic acid and oleic acid. These results indicate that AhFatA is at least partially responsible for determining the high palmitoleic acid and oleic acid composition of E. coli.

引用

页数：5

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