Automobile diesel exhaust particles induce lipid droplet formation in macrophages in vitro

被引：34

作者：

Cao, Yi ^{[1
]}

Jantzen, Kim ^{[1
]}

Gouveia, Ana Cecilia Damiao ^{[1
]}

Skovmand, Astrid ^{[1
]}

Roursgaard, Martin ^{[1
]}

Loft, Steffen ^{[1
]}

Moller, Peter ^{[1
]}

机构：

[1] Univ Copenhagen, Environm Hlth Sect, Dept Publ Hlth, DK-1014 Copenhagen K, Denmark

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2015年 / 40卷 / 01期

关键词：

Automobile diesel exhaust particles; Oxidative stress; Lysosomal dysfunction; Lipid droplet; OXIDATIVE STRESS; CHOLESTEROL EFFLUX; ENDOTHELIAL-CELLS; LYSOSOMAL DYSFUNCTION; POLLUTANT CHEMICALS; CARBON NANOTUBES; NANOPARTICLES; TOXICITY; EXPOSURE; DAMAGE;

D O I：

10.1016/j.etap.2015.06.012

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Exposure to diesel exhaust particles (DEP) has been associated with adverse cardiopulmonary health effects, which may be related to dysregulation of lipid metabolism and formation of macrophage foam cells. In this study, THP-1 derived macrophages were exposed to an automobile generated DEP (A-DEP) for 24 h to study lipid droplet formation and possible mechanisms. The results show that A-DEP did not induce cytotoxicity. The production of reactive oxygen species was only significantly increased after exposure for 3 h, but not 24h. Intracellular level of reduced glutathione was increased after 24h exposure. These results combined indicate an adaptive response to oxidative stress. Exposure to A-DEP was associated with significantly increased formation of lipid droplets, as well as changes in lysosomal function, assessed as reduced LysoTracker staining. In conclusion, these results indicated that exposure to A-DEP may induce formation of lipid droplets in macrophages in vitro possibly via lysosomal dysfunction. (C) 2015 Elsevier By. All rights reserved.

引用

页码：164 / 171

页数：8