Interaction of curcumin with intravenous immunoglobulin: A fluorescence quenching and Fourier transformation infrared spectroscopy study

被引：49

作者：

Liu, Yongchun ^{[1
,2
]}

Yang, Zhengyin ^{[1
]}

Du, Juan ^{[1
]}

Yao, Xiaojun ^{[1
]}

Lei, Ruixia ^{[2
]}

Zheng, Xudong ^{[2
]}

Liu, Jianning ^{[2
]}

Hu, Huaisheng ^{[2
]}

Li, Hong ^{[2
]}

机构：

[1] Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China

[2] Longdong Univ, Coll Chem & Chem Engn, Qingyang 745000, Gansu, Peoples R China

来源：

IMMUNOBIOLOGY | 2008年 / 213卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Curcumin; Fluorescence quenching; Fourier transformation infrared spectra (FTIR); Immune gamma globulin (IgG); Intravenous immunoglobulin (IVIG);

D O I：

10.1016/j.imbio.2008.02.003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The interaction of curcumin with intravenous immunoglobulin (IVIG) mainly composed of immune gamma globulin (IgG) was studied in vitro by spectroscopic methods including fluorescence spectroscopy and Fourier transformation infrared (FTIR) spectroscopy. Docking was used to calculate the interaction mode between curcumin and IVIG. The binding parameters for the reaction were calculated according to the Sips equation, which suggested that the binding of IVIG to curcumin was characterized by two binding sites with average affinity constant K-0 at 1.70 x 10(4) M-1 (296 K), and it was a non-specific and weak drug-protein interaction. The secondary structure compositions of free IVIG and its curcumin complexes were calculated by the FTIR difference spectra, self-deconvolution, second derivative resolution enhancement and the curve -fitting procedures of amide I band. The observed spectral changes indicate a partial unfolding of the protein structure, but the typical beta sturctural conformation of IVIG is still retained. The average binding distance between curcumin and the chromophore of IVIG (5.57 nm) was obtained using the theory of Forster energy transfer. IVIG can serve as transport protein (carrier) for curcumin. (C) 2008 Elsevier Gmbh. All rights reserved.

引用

页码：651 / 661

页数：11

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