Evaluation of METase-pemetrexed-loaded PEG-PLGA nanoparticles modified with anti-CD133-scFV for treatment of gastric carcinoma

被引：19

作者：

Xin, Lin ^{[1
]}

Zhang, Hou-Ting ^{[1
]}

Yang, Wei-Feng ^{[1
]}

Li, Yi-Fan ^{[1
]}

Liu, Chuan ^{[1
]}

机构：

[1] Nanchang Univ, Dept Gen Surg, Affiliated Hosp 2, Nanchang 330006, Jiangxi, Peoples R China

来源：

BIOSCIENCE REPORTS | 2018年 / 38卷

基金：

中国国家自然科学基金;

关键词：

CANCER STEM-CELLS; RECOMBINANT METHIONINASE; TREATING PATIENTS; DRUG-DELIVERY; GENE; LIPOSOMES; EFFICACY; CHEMORESISTANCE; IDENTIFICATION; CHEMOTHERAPY;

D O I：

10.1042/BSR20171001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

PEG-PLGA nanoparticles (NPs) modified with anti-CD133 and tumor-targeting single-chain antibody fragment (scFV-NPs) for systemic delivery of methioninase (METase) and pemetrexed for gastric carcinoma were successfully formulated. The structure characterization and biological functions of METase-pemetrexed-loaded scFV-PEG-PLGA NPs (scFV-METase/pemetrexed-NPs) in vitro were investigated. Functional scFV-PEG-PLGA NPs or PEG-PLGA NPs present low cell cytoxicity in CD133+ SGC7901 cells. scFV-METase/pemetrexed-NPs (scFv-M/P-NP) was more effective in inhibiting tumor growth (including cell growth and migration ability) in CD133 positive expressed gastric cancer cells than METase/pemetrexed-NPs (M/P-NP). Moreover, METase enhanced the inhibitory effect of pemetrexed on thymidylate synthase (TS) synthesis and cell apoptosis. We have demonstrated the application of scFV-targeted PEG-PLGA NPs as a new potential strategy to enhance treatment benefits for gastric carcinoma.

引用

页数：11

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