Mechanism of Action of Mangifera indica Leaves for Anti-Diabetic Activity

Diabetes is a major metabolic disorder whose prevalence is increasing daily. Medicinal plants have played an important role in the prevention and treatment of type 2 diabetes via prophylactic and therapeutic management. In this study, Mangifera Indica leaf (MIL) extract was investigated for its promising anti-diabetic activity via an in vitro model. It was found that MIL extract possessed significant inhibition on alpha-amylase activity up to (51.4 +/- 2.7)% at a concentration of 200 mu g/mL. Moreover, glucose adsorption capacity of MIL was identified at (2.7 +/- 0.19) mM glucose/g extract. Furthermore, the extract caused a significant increase in glucose uptake up to (143 +/- 9.3)% in LO-2 liver cells. Notably, MIL extract was effective in scavenging (63.3 +/- 2.1)% 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and (71.6 +/- 4.3)% 2,2-azinobis-3-ethyl benzothiazoline-6-sulfonic acid (ABTS)(+) radicals and inhibiting (66 +/- 4.9)% NO production from RAW264.7 cells without any cytotoxicity effects. Accordingly, M. indica leaves are suggested as a promising material for development of hypoglycemic products.