miRTCat: a comprehensive map of human and mouse microRNA target sites including non-canonical nucleation bulges

被引：13
作者：
Kim, Ka-Kyung [1 ,2 ]
Ham, Juyoung [2 ]
Chi, Sung Wook [1 ,2 ,3 ]
机构：
[1] Sungkyunkwan Univ, Samsung Biomed Res Inst, Seoul, South Korea
[2] Sungkyunkwan Univ, Dept Hlth Sci & Technol, Samsung Adv Inst Hlth Sci & Technol, Seoul, South Korea
[3] Samsung Med Ctr, Samsung Res Inst Future Med, Seoul 135710, South Korea
来源：
BIOINFORMATICS | 2013年 / 29卷 / 15期
关键词：
WIDE IDENTIFICATION; BINDING-SITES;
D O I：
10.1093/bioinformatics/btt296
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
MicroRNAs (miRNAs) regulate various biological functions by binding hundreds of transcripts to impart post-transcriptional repression. Recently, by applying a transcriptome-wide experimental method for identifying miRNA target sites (Ago HITS-CLIP), a novel non-canonical target site, named 'nucleation bulge', was discovered as widespread, functional and evolutionally conserved. Although such non-canonical nucleation bulges have been proven to be predictive by using 'pivot pairing rule' and sequence conservation, this approach has not been applied yet. To facilitate the functional studies of non-canonical miRNA targets, we implement miRTCat: a comprehensive searchable map of miRNA target sites, including non-canonical nucleation bulges, not only mapped in experimentally verified miRNA-bound regions but also predicted in all 30-untranslated regions (30-UTRs) derived from human and mouse (similar to 15.6% as expected false-positive results).
引用
收藏
页码：1898 / 1899
页数：2
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