Impedimetric detection of in situ interaction between anti-cancer drug bleomycin and DNA

被引：46

作者：

Erdem, Arzum ^{[1
]}

Congur, Gulsah ^{[1
]}

机构：

[1] Ege Univ, Fac Pharm, Dept Analyt Chem, TR-35100 Izmir, Turkey

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2013年 / 61卷

关键词：

Bleomycin; Drug-DNA interactions; Electrochemical impedance spectroscopy; Mitomycin C; Cis-platin; LIQUID-CHROMATOGRAPHIC METHOD; ENZYME-IMMUNOASSAY; NUCLEIC-ACIDS; MITOMYCIN-C; CLEAVAGE; BINDING; RADIOIMMUNOASSAY; QUANTIFICATION; SPECTROSCOPY; CHEMOTHERAPY;

D O I：

10.1016/j.ijbiomac.2013.07.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Surface confined interaction of anti-cancer drug bleomycin (BLM) with nucleic acids: single stranded and double stranded DNA was investigated herein by using electrochemical impedance spectroscopy (EIS) technique in combination with a graphite sensor technology. The experimental conditions were optimized: such as, dsDNA concentration, BLM concentration and interaction time. The main features of impedimetric DNA biosensor, such as its detection limit and the repeatability, were also discussed. The in situ interaction of BLM with dsDNA was also tested impedimetrically in the absence or presence of other chemotherapeutic agents, such as mitomycin C (MC) and cis-platin (cis-DDP) for testing the selectivity. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：295 / 301

页数：7

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