Batch and fed-batch growth of Pichia pastoris under increased air pressure

被引:7
作者
Lopes, Marlene [1 ]
Belo, Isabel [1 ]
Mota, Manuel [1 ]
机构
[1] Univ Minho, Inst Biotechnol & Bioengn IBB, Ctr Biol Engn, P-4710057 Braga, Portugal
关键词
Pichia pastoris; Increased air pressure; Oxygen transfer rate (OTR); Crude glycerol; ESCHERICHIA-COLI; OVERFLOW METABOLISM; PROTEIN EXPRESSION; OXYGEN-TRANSFER; CRUDE GLYCEROL; MASS-TRANSFER; YEAST; CULTURES; FERMENTATION; ENZYME;
D O I
10.1007/s00449-012-0871-5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Pichia pastoris CBS 2612 behavior under air pressures of 1, 3 and 5 bar in culture media of glycerol (pure and crude) and methanol was studied. Generally, the increase in oxygen transfer rate due to the increase of total pressure improved cellular growth for all carbon sources and for batch and fed-batch processes with different feeding rate strategies. In batch cultures, 1.4-, 1.2-, and 1.5-fold improvement in biomass production was obtained with the increase of air pressure up to 5 bar, using methanol, pure glycerol, and crude glycerol, respectively. The increase of air pressure to 5 bar using exponential feeding rate led to 1.4-fold improvement in biomass yield per glycerol mass consumed, for crude and pure glycerol. The current low cost of crude glycerol from the biodiesel production together with the present results shows the possibility of improving cell mass production of P. pastoris using increased air pressure.
引用
收藏
页码:1267 / 1275
页数:9
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