Comparative Proteomic Analysis of Aluminum Tolerance in Tibetan Wild and Cultivated Barleys

被引:28
作者
Dai, Huaxin [1 ]
Cao, Fangbin [1 ]
Chen, Xianhong [1 ]
Zhang, Mian [1 ]
Ahmed, Imrul Mosaddek [1 ]
Chen, Zhong-Hua [2 ]
Li, Chengdao [3 ]
Zhang, Guoping [1 ]
Wu, Feibo [1 ]
机构
[1] Zhejiang Univ, Dept Agron, Coll Agr & Biotechnol, Hangzhou 310003, Zhejiang, Peoples R China
[2] Univ Western Sydney, Sch Sci & Hlth, Penrith, NSW 1797, Australia
[3] Govt Western Australia, Dept Agr, S Perth, WA, Australia
来源
PLOS ONE | 2013年 / 8卷 / 05期
基金
中国国家自然科学基金;
关键词
ADENOSYLMETHIONINE SYNTHETASE GENE; CITRATE SECRETION; OXIDATIVE STRESS; EXPRESSION; RESISTANCE; ARABIDOPSIS; PLANTS; ACID; BIOSYNTHESIS; ACCUMULATION;
D O I
10.1371/journal.pone.0063428
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Aluminum (Al) toxicity is a major limiting factor for plant production in acid soils. Wild barley germplasm is rich in genetic diversity and may provide elite genes for crop Al tolerance improvement. The hydroponic-experiments were performed to compare proteomic and transcriptional characteristics of two contrasting Tibetan wild barley genotypes Al-resistant/tolerant XZ16 and Al-sensitive XZ61 as well as Al-resistant cv. Dayton. Results showed that XZ16 had less Al uptake and translocation than XZ61 and Dayton under Al stress. Thirty-five Al-tolerance/resistance-associated proteins were identified and categorized mainly in metabolism, energy, cell growth/division, protein biosynthesis, protein destination/storage, transporter, signal transduction, disease/defense, etc. Among them, 30 were mapped on barley genome, with 16 proteins being exclusively up-regulated by Al stress in XZ16, including 4 proteins (S-adenosylmethionine-synthase 3, ATP synthase beta subunit, triosephosphate isomerase, Bp2A) specifically expressed in XZ16 but not Dayton. The findings highlighted the significance of specific-proteins associated with Al tolerance, and verified Tibetan wild barley as a novel genetic resource for Al tolerance.
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页数:14
相关论文
共 47 条
[1]  
[Anonymous], 1997, PRACTICAL STAT ITS D
[2]   Comparative proteomic analysis of Typha angustifolia leaf under chromium, cadmium and lead stress [J].
Bah, Alieu Mohamed ;
Sun, Hongyan ;
Chen, Fei ;
Zhou, Jing ;
Dai, Huaxin ;
Zhang, Guoping ;
Wu, Feibo .
JOURNAL OF HAZARDOUS MATERIALS, 2010, 184 (1-3) :191-203
[3]   Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana [J].
Bevan, M ;
Bancroft, I ;
Bent, E ;
Love, K ;
Goodman, H ;
Dean, C ;
Bergkamp, R ;
Dirkse, W ;
Van Staveren, M ;
Stiekema, W ;
Drost, L ;
Ridley, P ;
Hudson, SA ;
Patel, K ;
Murphy, G ;
Piffanelli, P ;
Wedler, H ;
Wedler, E ;
Wambutt, R ;
Weitzenegger, T ;
Pohl, TM ;
Terryn, N ;
Gielen, J ;
Villarroel, R ;
De Clerck, R ;
Van Montagu, M ;
Lecharny, A ;
Auborg, S ;
Gy, I ;
Kreis, M ;
Lao, N ;
Kavanagh, T ;
Hempel, S ;
Kotter, P ;
Entian, KD ;
Rieger, M ;
Schaeffer, M ;
Funk, B ;
Mueller-Auer, S ;
Silvey, M ;
James, R ;
Montfort, A ;
Pons, A ;
Puigdomenech, P ;
Douka, A ;
Voukelatou, E ;
Milioni, D ;
Hatzopoulos, P ;
Piravandi, E ;
Obermaier, B .
NATURE, 1998, 391 (6666) :485-488
[4]   Preparation of protein extracts from recalcitrant plant tissues: An evaluation of different methods for two-dimensional gel electrophoresis analysis [J].
Carpentier, SC ;
Witters, E ;
Laukens, K ;
Deckers, P ;
Swennen, R ;
Panis, B .
PROTEOMICS, 2005, 5 (10) :2497-2507
[5]   Difference in response to aluminum stress among Tibetan wild barley genotypes [J].
Dai, Huaxin ;
Shan, Wunian ;
Zhao, Jing ;
Zhang, Guoping ;
Li, Chengdao ;
Wu, Feibo .
JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE, 2011, 174 (06) :952-960
[6]   Three proliferating cell nuclear antigen-like proteins found in the hyperthermophilic archaeon Aeropyrum pernix:: Interactions with the two DNA polymerases [J].
Daimon, K ;
Kawarabayasi, Y ;
Kikuchi, H ;
Sako, Y ;
Ishino, Y .
JOURNAL OF BACTERIOLOGY, 2002, 184 (03) :687-694
[7]   DIFFERENTIAL ACCUMULATION OF S-ADENOSYLMETHIONINE SYNTHETASE TRANSCRIPTS IN RESPONSE TO SALT STRESS [J].
ESPARTERO, J ;
PINTORTORO, JA ;
PARDO, JM .
PLANT MOLECULAR BIOLOGY, 1994, 25 (02) :217-227
[8]  
Flores H.E., 1989, plant Nitrogen Metabolism, P329, DOI 10.1007/978-1-4613-0835-5_10
[9]   POSTTRANSCRIPTIONAL AND POSTTRANSLATIONAL CONTROL OF ENOLASE EXPRESSION IN THE FACULTATIVE CRASSULACEAN ACID METABOLISM PLANT MESEMBRYANTHEMUM-CRYSTALLINUM L [J].
FORSTHOEFEL, NR ;
CUSHMAN, MAF ;
CUSHMAN, JC .
PLANT PHYSIOLOGY, 1995, 108 (03) :1185-1195
[10]   Tolerance of barley cultivars to an acid, aluminum-toxic subsoil related to mineral element concentrations in their shoots [J].
Foy, CD .
JOURNAL OF PLANT NUTRITION, 1996, 19 (10-11) :1361-1380