Microplate alamarBlue Assay for Paracoccidioides Susceptibility Testing

被引：39
作者：
de Paula e Silva, A. C. A.
Oliveira, H. C.
Silva, J. F.
Sangalli-Leite, F.
Scorzoni, L.
Fusco-Almeida, A. M.
Mendes-Giannini, M. J. S. [1 ]
机构：
[1] UNESP Univ Estadual Paulista, Fac Ciencias Farmaceut, Araraquara, Brazil
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2013年 / 51卷 / 04期
基金：
巴西圣保罗研究基金会;
关键词：
HUMAN-IMMUNODEFICIENCY-VIRUS; RESAZURIN MICROTITER ASSAY; ANTIFUNGAL ACTIVITY; BRASILIENSIS; EXTRACTS;
D O I：
10.1128/JCM.02914-12
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
CLSI method M27-A3 is not available for use with dimorphic fungi, such as those of the Paracoccidioides genus. In this study, we developed a microdilution method and added the alamarBlue reagent to test the responses of Paracoccidioides brasiliensis and Paracoccidioides lutzii against amphotericin B and itraconazole antifungals. The test proved to be sensitive, practical, and inexpensive and can be used to monitor the activity of low-growth microorganisms and their response to various drugs.
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收藏
页码：1250 / 1252
页数：3
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