Lactobacilli Regulate Staphylococcus aureus 161:2-Induced Pro-Inflammatory T-Cell Responses In Vitro

被引:14
作者
Haileselassie, Yeneneh [1 ]
Johansson, Maria A. [1 ]
Zimmer, Christine L. [1 ]
Bjorkander, Sophia [1 ]
Petursdottir, Dagbjort H. [1 ]
Dicksved, Johan [2 ]
Petersson, Mikael [3 ]
Persson, Jan-Olov [3 ]
Fernandez, Carmen [1 ]
Roos, Stefan [4 ]
Holmlund, Ulrika [1 ]
Sverremark-Ekstrom, Eva [1 ]
机构
[1] Stockholm Univ, Dept Mol Biosci, Wenner Gren Inst, S-10691 Stockholm, Sweden
[2] Swedish Univ Agr Sci, Dept Anim Nutr & Management, Uppsala, Sweden
[3] Stockholm Univ, Dept Math, Div Math Stat, S-10691 Stockholm, Sweden
[4] Swedish Univ Agr Sci, Dept Microbiol, Uppsala, Sweden
基金
瑞典研究理事会;
关键词
BLOOD MONONUCLEAR-CELLS; INTESTINAL MICROFLORA; DENDRITIC CELLS; IMMUNE-RESPONSES; GUT MICROBIOTA; BACTERIA; HEALTHY; BIFIDOBACTERIUM; ALLERGY; GENE;
D O I
10.1371/journal.pone.0077893
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
There seems to be a correlation between early gut microbiota composition and postnatal immune development. Alteration in the microbial composition early in life has been associated with immune mediated diseases, such as autoimmunity and allergy. We have previously observed associations between the presence of lactobacilli and Staphylococcus (S.) aureus in the early-life gut microbiota, cytokine responses and allergy development in children. Consistent with the objective to understand how bacteria modulate the cytokine response of intestinal epithelial cell (IEC) lines and immune cells, we exposed IEC lines (HT29, SW480) to UV-killed bacteria and/or culture supernatants (-sn) from seven Lactobacillus strains and three S. aureus strains, while peripheral blood mononuclear cells (PBMC) and cord blood mononuclear cells (CBMC) from healthy donors were stimulated by bacteria-sn or with bacteria conditioned IEC-sn. Although the overall IEC response to bacterial exposure was characterized by limited sets of cytokine and chemokine production, S. aureus 161: 2-sn induced an inflammatory response in the IEC, characterized by CXCL1/GROa and CXCL8/IL-8 production, partly in a MyD88-dependent manner. UV-killed bacteria did not induce a response in the IEC line, and a combination of both UV-killed bacteria and the bacteria-sn had no additive effect to that of the supernatant alone. In PBMC, most of the Lactobacillus-sn and S. aureus-sn strains were able to induce a wide array of cytokines, but only S. aureus-sn induced the T-cell associated cytokines IL-2, IL-17 and IFN-gamma, independently of IEC-produced factors, and induced up regulation of CTLA-4 expression and IL-10 production by T-regulatory cells. Notably, S. aureus-sn-induced T-cell production of IFN-gamma and IL-17 was down regulated by the simultaneous presence of any of the different Lactobacillus strains, while the IEC CXCL8/IL-8 response was unaltered. Thus these studies present a possible role for lactobacilli in induction of immune cell regulation, although the mechanisms need to be further elucidated.
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页数:12
相关论文
共 45 条
[1]   Neonatal immune responses to microbial stimuli:: Is there an influence of maternal allergy? [J].
Amoudruz, P ;
Holmlund, U ;
Malmström, V ;
Trollmo, C ;
Bremme, K ;
Scheynius, A ;
Sverremark-Ekström, E .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2005, 115 (06) :1304-1310
[2]   ATP drives lamina propria TH17 cell differentiation [J].
Atarashi, Koji ;
Nishimura, Junichi ;
Shima, Tatsuichiro ;
Umesaki, Yoshinori ;
Yamamoto, Masahiro ;
Onoue, Masaharu ;
Yagita, Hideo ;
Ishii, Naoto ;
Evans, Richard ;
Honda, Kenya ;
Takeda, Kiyoshi .
NATURE, 2008, 455 (7214) :808-U10
[3]   Unlocking the ultrastructure of colorectal cancer cells in vitro using selective staining [J].
Biazik, Joanna M. ;
Jahn, Kristina A. ;
Su, Yingying ;
Wu, Ya-Na ;
Braet, Filip .
WORLD JOURNAL OF GASTROENTEROLOGY, 2010, 16 (22) :2743-2753
[4]   Allergy development and the intestinal microflora during the first year of life [J].
Björkstén, B ;
Sepp, E ;
Julge, K ;
Voor, T ;
Mikelsaar, M .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2001, 108 (04) :516-520
[5]  
Björkstén B, 1999, CLIN EXP ALLERGY, V29, P342, DOI 10.1046/j.1365-2222.1999.00560.x
[6]  
BJORNEHOLM S, 2011, MICROB ECOL HLTH DIS, V14, P57564
[7]   Enterotoxin-producing staphylococci cause intestinal inflammation by a combination of direct epithelial cytopathy and superantigen-mediated T-cell activation [J].
Edwards, Lindsey A. ;
O'Neill, Colette ;
Furman, Mark A. ;
Hicks, Susan ;
Torrente, Franco ;
Perez-Machado, Miguel ;
Wellington, Elizabeth M. ;
Phillips, Alan D. ;
Murch, Simon H. .
INFLAMMATORY BOWEL DISEASES, 2012, 18 (04) :624-640
[8]   The function of TLR2 during staphylococcal diseases [J].
Fournier, Benedicte .
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2013, 3
[9]   Effects of probiotic bacteria and their genomic DNA on TH1/TH2-cytokine production by peripheral blood mononuclear cells (PBMCs) of healthy and allergic subjects [J].
Ghadimi, Darab ;
Foelster-Holst, Regina ;
de Vrese, Michael ;
Winkler, Petra ;
Heller, Knut J. ;
Schrezenmeir, Juergen .
IMMUNOBIOLOGY, 2008, 213 (08) :677-692
[10]   Maternal country of origin, breast milk characteristics and potential influences on immunity in offspring [J].
Holmlund, U. ;
Amoudruz, P. ;
Johansson, M. A. ;
Haileselassie, Y. ;
Ongoiba, A. ;
Kayentao, K. ;
Traore, B. ;
Doumbo, S. ;
Schollin, J. ;
Doumbo, O. ;
Montgomery, S. M. ;
Sverremark-Ekstroem, E. .
CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 2010, 162 (03) :500-509