Hypoculoside, a sphingoid base-like compound from Acremonium disrupts the membrane integrity of yeast cells

被引：10

作者：

Alfatah, Mohammad ^{[1
]}

Wong, Jin Huei ^{[1
]}

Nge, Choy E. Ng ^{[1
]}

Kong, Kiat Whye ^{[2
]}

Low, Kia Ngee ^{[1
]}

Leong, Chung Yan ^{[1
]}

Crasta, Sharon ^{[1
]}

Munusamy, Madhaiyan ^{[1
]}

Chang, Amanda Mun Leng ^{[1
]}

Hoon, Shawn ^{[2
]}

Ng, Siew Bee ^{[1
]}

Kanagasundaram, Yoganathan ^{[1
]}

Arumugam, Prakash ^{[1
]}

机构：

[1] Bioinformat Inst, 30 Biopolis St,07-01 Matrix, Singapore 138671, Singapore

[2] Mol Engn Lab, 61 Biopolis Dr,03-12 Proteos, Singapore 13867, Singapore

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

关键词：

FUMONISIN BIOSYNTHESIS; PATHWAY; SPHINGANINE; APOPTOSIS; MUTANTS; FUM13;

D O I：

10.1038/s41598-018-35979-z

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have isolated Hypoculoside, a new glycosidic amino alcohol lipid from the fungus Acremonium sp. F2434 belonging to the order Hypocreales and determined its structure by 2D-NMR (Nuclear Magnetic Resonance) spectroscopy. Hypoculoside has antifungal, antibacterial and cytotoxic activities. Homozygous profiling (HOP) of hypoculoside in Saccharomyces cerevisiae (budding yeast) revealed that several mutants defective in vesicular trafficking and vacuolar protein transport are sensitive to hypoculoside. Staining of budding yeast cells with the styryl dye FM4-64 indicated that hypoculoside damaged the vacuolar structure. Furthermore, the propidium iodide (PI) uptake assay showed that hypoculoside disrupted the plasma membrane integrity of budding yeast cells. Interestingly, the glycosidic moiety of hypoculoside is required for its deleterious effect on growth, vacuoles and plasma membrane of budding yeast cells.

引用

页数：16

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