Apoptosis is involved in the mechanism of postresuscitation myocardial dysfunction in a porcine model of cardiac arrest

被引:22
作者
Gu, Wei [1 ]
Li, Chun Sheng [1 ]
Yin, Wen Peng [1 ]
Guo, Zhi Jun [1 ]
Hou, Xiao Min [1 ]
Zhang, Da [1 ]
机构
[1] Capital Med Univ, Beijing Chaoyang Hosp, Dept Emergency Med, Beijing, Peoples R China
关键词
VENTRICULAR-FIBRILLATION; RESUSCITATION; CARDIOPULMONARY; HYPOTHERMIA; CASPASE-3; HEART;
D O I
10.1016/j.ajem.2012.04.031
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
Background: Postresuscitation myocardial dysfunction contributes to the low survival rate after successful resuscitation, but its mechanism remains poorly understood. This study investigated whether caspase 3-mediated apoptosis is activated in the heart after postresuscitation myocardial dysfunction. Methods: After pigs were subjected to 8 minutes of electrically induced cardiac arrest (CA), standard cardiopulmonary resuscitation was performed. Animals in the post-return of spontaneous circulation (ROSC) group were randomly assigned to be killed (n = 6 per group) at 12 and 24 hours after ROSC, and myocardial specimens were analyzed with electron microscopy, Western blotting, quantitative real-time polymerase chain reaction, and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay. Results: Myocardial function was significantly impaired after ROSC. Expression of Bcl-2, Bax, and caspase 3 protein was markedly increased in the post-ROSC group compared with the sham-operated group (P < .05) at 12 and 24 hours after ROSC, whereas Bcl-2/Bax was significantly reduced in the post-ROSC group compared with the sham-operated group (P < .05). The messenger RNA levels of caspase 3 were significantly elevated at 12 and 24 hours after ROSC, and increases in caspase 3 activity indicated activation of the mitochondrial apoptotic pathway. Typical apoptotic nuclei were observed in cardiomyocytes 24 hours after ROSC. More apoptotic cells were observed in animals that had undergone CA compared with sham-operated animals (P < .05). Conclusion: Caspase 3-mediated apoptosis may be one of the main pathologic mechanisms of postresuscitation myocardial injury in a porcine model of CA. (C) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码:2039 / 2045
页数:7
相关论文
共 50 条
  • [41] Prolonged cardiac arrest: A revised model of porcine ventricular fibrillation
    Mader, Timothy J.
    RESUSCITATION, 2008, 76 (03) : 481 - 484
  • [42] Resuscitation of Exsanguination Cardiac Arrest in a Porcine Model Using REBOA
    Simovic, Milomir
    CIRCULATION, 2017, 136 (24) : E466 - E466
  • [43] Attenuating the defibrillation dosage decreases postresuscitation myocardial dysfunction in a swine model of pediatric ventricular fibrillation
    Berg, Marc D.
    Banville, Isabelle L.
    Chapman, Fred W.
    Walker, Robert G.
    Gaballa, Mohammed A.
    Hilwig, Ronald W.
    Samson, Ricardo A.
    Kern, Karl B.
    Berg, Robert A.
    PEDIATRIC CRITICAL CARE MEDICINE, 2008, 9 (04) : 429 - 434
  • [44] Urocortin Treatment Improves Acute Hemodynamic Instability and Reduces Myocardial Damage in Post-Cardiac Arrest Myocardial Dysfunction
    Huang, Chien-Hua
    Wang, Chih-Hung
    Tsai, Min-Shan
    Hsu, Nai-Tan
    Chiang, Chih-Yen
    Wang, Tzung-Dau
    Chang, Wei-Tien
    Chen, Huei-Wen
    Chen, Wen-Jone
    PLOS ONE, 2016, 11 (11):
  • [45] Risk Factor Analyses for the Return of Spontaneous Circulation in the Asphyxiation Cardiac Arrest Porcine Model
    Wu, Cai-Jun
    Guo, Zhi-Jun
    Li, Chun-Sheng
    Zhang, Yi
    Yang, Jun
    CHINESE MEDICAL JOURNAL, 2015, 128 (08) : 1096 - 1101
  • [46] Effect of mild hypercapnia on outcome and histological injury in a porcine post cardiac arrest model
    Babini, Giovanni
    Ristagno, Giuseppe
    Boccardo, Antonio
    De Giorgio, Daria
    De Maglie, Marcella
    Affatato, Roberta
    Ceriani, Sabina
    Zani, Davide
    Novelli, Deborah
    Staszewsky, Lidia
    Masson, Serge
    Pravettoni, Davide
    Latini, Roberto
    Belloli, Angelo
    Scanziani, Eugenio
    Skrifvars, Markus
    RESUSCITATION, 2019, 135 : 110 - 117
  • [47] Relationship between post-cardiac arrest myocardial oxidative stress and myocardial dysfunction in the rat
    Hackenhaar, Fernanda Schaefer
    Fumagalli, Francesca
    Li Volti, Giovanni
    Sorrenti, Valeria
    Russo, Ilaria
    Staszewsky, Lidia
    Masson, Serge
    Latini, Roberto
    Ristagno, Giuseppe
    JOURNAL OF BIOMEDICAL SCIENCE, 2014, 21
  • [48] Ultrafast Cooling With Total Liquid Ventilation Mitigates Early Inflammatory Response and Offers Neuroprotection in a Porcine Model of Cardiac Arrest
    Abi Zeid Daou, Yara
    Watanabe, Naoto
    Lidouren, Fanny
    Bois, Antoine
    Faucher, Estelle
    Huet, Helene
    Hutin, Alice
    Jendoubi, Ali
    Surenaud, Mathieu
    Hue, Sophie
    Nadeau, Mathieu
    Perrotto, Sandrine
    Libardi, Mickael
    Ghaleh, Bijan
    Micheau, Philippe
    Bruneval, Patrick
    Cariou, Alain
    Kohlhauer, Matthias
    Tissier, Renaud
    JOURNAL OF THE AMERICAN HEART ASSOCIATION, 2024, 13 (16):
  • [49] Adrenaline has a limited effect on myocardial microvascular blood flow: A randomised experimental study in a porcine cardiac arrest model
    Wagner, Henrik
    Mlcek, Mikulas
    Krupickova, Petra
    Popkova, Michaela
    Mejstrik, Alan
    Boucek, Tomas
    Michalek, Pavel
    Kittnar, Otomar
    Belohlavek, Jan
    RESUSCITATION PLUS, 2025, 22
  • [50] Brain and Myocardial Mitochondria Follow Different Patterns of Dysfunction After Cardiac Arrest
    Kohlhauer, Matthias
    Panel, Mathieu
    des Roches, Marine Vermot
    Faucher, Estelle
    Daou, Yara Abi Zeid
    Boissady, Emilie
    Lidouren, Fanny
    Ghaleh, Bijan
    Morin, Didier
    Tissier, Renaud
    SHOCK, 2021, 56 (05): : 857 - 864