A first insight on the population structure of Mycobacterium tuberculosis complex as studied by spoligotyping and MIRU-VNTRs in Bogota, Colombia

被引:44
作者
Cerezo, Irene [1 ]
Jimenez, Yesica [1 ]
Hernandez, Johana [1 ]
Zozio, Thierry [2 ]
Murcia, Martha I. [1 ]
Rastogi, Nalin [2 ]
机构
[1] Univ Nacl Colombia, Fac Med, Dept Microbiol, Bogota, Colombia
[2] Inst Pasteur Guadeloupe, Unite TB & Mycobacteries, WHO Supranat TB Reference Lab, Abymes, Guadeloupe, France
关键词
Mycobacterium tuberculosis; Population structure; Spoligotyping; MIRU-VNTRs; Bogota; Colombia; MOLECULAR EPIDEMIOLOGY; GENETIC DIVERSITY; DNA; STRAINS; UTILITY;
D O I
10.1016/j.meegid.2011.07.006
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
With an incidence of 25.6/100,000 in 2008, tuberculosis (TB) remains an important public health problem in Colombia. In this study, a total of 152 Mycobacterium tuberculosis complex strains isolated in Bogota, Colombia between years 1995 and 2007 were genotyped by spoligotyping and 12-loci MIRU-VNTRs. The various spoligotyping-based genotypic lineages in our sample were: Latin American & Mediterranean (LAM) n = 75, 49.34%; Haarlem, n = 38, 25.0%; ill-defined T group, n = 21, 13.82%; S family, n = 5.3.29%; X clade, n = 2, 1.32%; Beijing, n = 1, 0.65%, while strains with unknown signatures (n = 10) represented 6.58% of isolates. Using spoligotyping as a first molecular marker and MIRU-VNTRs as second marker, we obtained 102 single patterns and 14 clustered patterns (n = 52 strains from 49 patients, 2-8 strains per cluster). The MIRU-VNTRs patterns corresponded to 50 MITs for 109 strains and 43 orphan patterns. The most frequent patterns were MIT190 (n = 12), MIT45 (n = 10), and MIT25 (n = 9). The Hunter & Gaston discriminatory index (HGDI) of both methodologies used together showed a value of 0.992. In our setting, the HGDI of five loci subset (MIRU10, 16, 23, 26 and 40) contributed most to the discriminatory power of 12-loci format used (HGDI = 0.977). The lineage distribution of M. tuberculosis showed that more than 3/4 of strains in Bogota are commonly found in Latin America, Caribbean, and Europe. This observation might reflect the shared post-Columbus history of Colombia and its Latin-American neighbors as well as strains brought in by 20th century immigrants from Europe. We also demonstrate the usefulness of MIRU-VNTR to detect suspected links among patients and polyclonal infections. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:657 / 663
页数:7
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