A Cell-Penetrating Ratiometric Nanoprobe for Intracellular Chloride

被引：21

作者：

Bau, Luca ^{[2
]}

Selvestrel, Francesco ^{[2
]}

Arduini, Maria ^{[2
]}

Zamparo, Ilaria ^{[1
]}

Lodovichi, Claudia ^{[1
]}

Mancin, Fabrizio ^{[2
]}

机构：

[1] VIMM, I-35129 Padua, Italy

[2] Univ Padua, Dipartimento Sci Chim, I-35131 Padua, Italy

来源：

ORGANIC LETTERS | 2012年 / 14卷 / 12期

基金：

欧洲研究理事会;

关键词：

SENSITIVE FLUORESCENT INDICATORS; SILICA NANOPARTICLES; MOLECULAR-BASIS; TRANSPORT; CHANNELS; SENSORS; CL;

D O I：

10.1021/ol300086w

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

NanoChlor, a nanoparticle-based fluorescent probe for chloride that is both ratiometric and capable of spontaneously penetrating neuronal cells at submillimolar concentrations, was designed and studied. NanoChlor is built on silica nanoparticles grafted with 6-methoxyquinolinium as the chloride-sensitive component and fluorescein as the reference dye. A Stern-Volmer constant of 50 M-1 was measured in Ringer's buffer at pH 7.2, and the response to chemically induced chloride currents was recorded in real time in hippocampal cells.

引用

页码：2984 / 2987

页数：4

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