Kinocilia Mediate Mechanosensitivity in Developing Zebrafish Hair Cells

被引:134
作者
Kindt, Katie S. [1 ]
Finch, Gabriel [1 ]
Nicolson, Teresa [1 ]
机构
[1] Oregon Hlth & Sci Univ, Howard Hughes Med Inst, Oregon Hearing Res Ctr, Portland, OR 97239 USA
关键词
LATERAL-LINE ORGAN; SENSORY TRANSDUCTION; INNER-EAR; TIP LINKS; MECHANOTRANSDUCER CHANNEL; DEVELOPMENTAL ACQUISITION; PRIMARY CILIUM; MOUSE COCHLEA; STEREOCILIA; CADHERIN-23;
D O I
10.1016/j.devcel.2012.05.022
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Mechanosensitive cilia are vital to signaling and development across many species. In sensory hair cells, sound and movement are transduced by apical hair bundles. Each bundle is comprised of a single primary cilium (kinocilium) flanked by multiple rows of actin-filled projections (stereocilia). Extracellular tip links that interconnect stereocilia are thought to gate mechanosensitive channels. In contrast to stereocilia, kinocilia are not critical for hair-cell mechanotransduction. However, by sequentially imaging the structure of hair bundles and mechanosensitivity of individual lateral-line hair cells in vivo, we uncovered a central role for kinocilia in mechanosensation during development. Our data demonstrate that nascent hair cells require kinocilia and kinocilial links for mechanosensitivity. Although nascent hair bundles have correct planar polarity, the polarity of their responses to mechanical stimuli is initially reversed. Later in development, a switch to correctly polarized mechanosensitivity coincides with the formation of tip links and the onset of tip-link-dependent mechanotransduction.
引用
收藏
页码:329 / 341
页数:13
相关论文
共 52 条
[1]   The tip-link antigen, a protein associated with the transduction complex of sensory hair cells, is protocadherin-15 [J].
Ahmed, Zubair M. ;
Goodyear, Richard ;
Riazuddin, Saima ;
Lagziel, Ayala ;
Legan, P. Kevin ;
Behra, Martine ;
Burgess, Shawn M. ;
Lilley, Kathryn S. ;
Wilcox, Edward R. ;
Riazuddin, Sheikh ;
Griffith, Andrew J. ;
Frolenkov, Gregory I. ;
Belyantseva, Inna A. ;
Richardson, Guy P. ;
Friedman, Thomas B. .
JOURNAL OF NEUROSCIENCE, 2006, 26 (26) :7022-7034
[2]   TIP-LINK INTEGRITY AND MECHANICAL TRANSDUCTION IN VERTEBRATE HAIR-CELLS [J].
ASSAD, JA ;
SHEPHERD, GMG ;
COREY, DP .
NEURON, 1991, 7 (06) :985-994
[3]   Localization of inner hair cell mechanotransducer channels using high-speed calcium imaging [J].
Beurg, Maryline ;
Fettiplace, Robert ;
Nam, Jong-Hoon ;
Ricci, Anthony J. .
NATURE NEUROSCIENCE, 2009, 12 (05) :553-558
[4]   Calcium imaging of single stereocilia in hair cells: Localization of transduction channels at both ends of tip links [J].
Denk, W ;
Holt, JR ;
Shepherd, GMG ;
Corey, DP .
NEURON, 1995, 15 (06) :1311-1321
[5]   Establishment of hair bundle polarity and orientation in the developing vestibular system of the mouse [J].
Denman-Johnson, K ;
Forge, A .
JOURNAL OF NEUROCYTOLOGY, 1999, 28 (10-11) :821-835
[6]  
ENGSTROM HANS, 1962, JOUR ACOUSTICAL SOC AMER, V34, P1356
[7]   Probing the pore of the auditory hair cell mechanotransducer channel in turtle [J].
Farris, HE ;
LeBlanc, CL ;
Goswami, J ;
Ricci, AJ .
JOURNAL OF PHYSIOLOGY-LONDON, 2004, 558 (03) :769-792
[8]   Molecular evolution of the vertebrate mechanosensory cell and ear [J].
Fritzsch, Bernd ;
Beisel, Kirk W. ;
Pauley, Sarah ;
Soukup, Garrett .
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, 2007, 51 (6-7) :663-678
[9]   Mechanoreception in motile flagella of Chlamydomonas [J].
Fujiu, Kenta ;
Nakayama, Yoshitaka ;
Iida, Hidetoshi ;
Sokabe, Masahiro ;
Yoshimura, Kenjiro .
NATURE CELL BIOLOGY, 2011, 13 (05) :630-U304
[10]   FM1-43 dye behaves as a permeant blocker of the hair-cell mechanotransducer channel [J].
Gale, JE ;
Marcotti, W ;
Kennedy, HJ ;
Kros, CJ ;
Richardson, GP .
JOURNAL OF NEUROSCIENCE, 2001, 21 (18) :7013-7025