Isozyme and RAPD markers linked to fertility restoring gene for cytoplasmic male sterile Allium fistulosum L. with cytoplasm of A-galanthum Kar. et Kir.

To develop the genetic markers linked to the fertility restoring gene (Rf) found in continuous backcross progenies between Allium gaianihum Kar. et Kir. (cytoplasm donor) and A. fistulosum L., isozymes and RAPDs were analyzed in the male fertile and male sterile plants in the backcross progenies (B-3 similar to B-5). in isozyme analysis of six enzymes which showed interspecific polymorphisms between A. galanthum and A. fistulosum, all the male fertile plants in the B-3, B-4, and B-5 generations had both alleles of A. galanthum and A. fistulosum at the phosphoglucoisomerase isozyme gene locus Pgi - 1, whereas all the male sterile plants had only the allele of A. fistulosum. These results indicate that the Pgi - 1 of A. galanthum is linked to the Rf locus. In RAPD analysis using 27 random decamer primers which showed interspecific polymorphisms between A. galanthum and A. fistulosum, all the male fertile plants had an A. galanthum-specific band (OPE03(700)), whereas none of the male sterile plants had this band. These results indicate that the OPE03(700) is linked to the Rf locus. These two genetic markers developed in this study will be useful to identify the genotypes at the Rf locus.