Properties of Lactobacillus reuteri chitosan-calcium-alginate encapsulation under simulated gastrointestinal conditions

被引：14
作者：
Huang, Hui-Ying [1 ]
Tang, Yi-Ju [1 ]
King, V. An-Erl [2 ]
Chou, Jen-Wei [3 ]
Tsen, Jen-Horng [1 ]
机构：
[1] China Med Univ, Dept Nutr, Taichung 404, Taiwan
[2] Natl Chung Hsing Univ, Dept Food Sci & Biotechnol, Taichung 40227, Taiwan
[3] China Med Univ, Coll Med, China Med Univ Hosp, Div Gastroenterol & Hepatol,Dept Internal Med, Taichung, Taiwan
来源：
INTERNATIONAL MICROBIOLOGY | 2015年 / 18卷 / 01期
关键词：
Lactobacillus reuteri; Listeria monocytogenes; chitosan-calcium-alginate encapsulation; probiotic properties; simulated gastrointestinal conditions; LACTIC-ACID BACTERIA; PROBIOTIC LACTOBACILLI; INTESTINAL MUCUS; ADHESION; SURVIVAL; PATHOGENS; STRAINS; CELLS; BEADS; PROTEIN;
D O I：
10.2436/20.1501.01.235
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The protective effects of encapsulation on the survival of Lactobacillus reuteri and the retention of the bacterium's probiotic properties under simulated gastrointestinal conditions were investigated. Viable counts and the remaining probiotic properties of calcium (Ca)-alginate encapsulated (A group), chitosan-Ca-alginate encapsulated (CA group), and unencapsulated, free L. reuteri (F group) were determined. Encapsulation improved the survival of L. reuteri subjected to simulated gastrointestinal conditions, with the greatest protective effect achieved in the CA group. The degree of cell membrane injury increased with increasing bile salt concentrations at constant pH, but the extent of injury was less in the encapsulated than in the free cells. Adherence rates were, in descending order: CA (0.524%) > A (0.360%) > F (0.275%). Lactobacillus reuteri cells retained their antagonistic activity toward Listeria monocytogenes even after incubation of the lactobacilli under simulated gastrointestinal conditions. Displacement of the pathogen by cells released from either of the encapsulation matrices was higher than that by free cells. The safety of L. reuteri was demonstrated in an in vitro invasion assay.
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收藏
页码：61 / 69
页数：9
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