Structural determination and pro-angiogenic effect of polysaccharide from the pollen of Typha angustifolia L.

被引：17

作者：

Gao, Mingliang ^{[1
]}

Lan, Jinshan ^{[1
]}

Zha, Yuling ^{[1
]}

Yao, Weifeng ^{[1
]}

Bao, Beihua ^{[1
]}

Shan, Mingqiu ^{[1
]}

Zhang, Fang ^{[1
,2
]}

Zhou, Guisheng ^{[1
,2
]}

Yu, Sheng ^{[1
]}

Cheng, Fangfang ^{[1
]}

Cao, Yudan ^{[1
]}

Yan, Hui ^{[1
,2
]}

Zhang, Li ^{[1
,2
,3
]}

Chen, Peidong ^{[1
,2
,3
]}

机构：

[1] Nanjing Univ Chinese Med, Sch Pharm, Nanjing 210023, Peoples R China

[2] Nanjing Univ Chinese Med, Jiangsu Collaborat Innovat Ctr Chinese Med Resourc, Sch Pharm, Nanjing 210023, Peoples R China

[3] Nanjing Univ Chinese Med, Xianlin Rd 138, Nanjing 210023, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2022年 / 222卷

基金：

中国国家自然科学基金;

关键词：

Polysaccharide; Typha angustifolia L; Structural determination; Pro-angiogenic; VEGF; PI3K; Akt signaling pathway; IN-VITRO; ANTIOXIDANT ACTIVITY; GROWTH-FACTOR; NITRIC-OXIDE; PURIFICATION; IDENTIFICATION; EXTRACTION; MANAGEMENT; STANDARDS; ACIDS;

D O I：

10.1016/j.ijbiomac.2022.10.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Four fractions of polysaccharides (TPP-1, TPP-2, TPP-3, and TPP-4) were isolated and purified from the pollen of Typha angustifolia L., and the structure of TPP-3 was furtherly determined by HPGPC (High Performance Gel Permeation Chromatography), monosaccharide composition analysis, methylation analysis and NMR (Nuclear Magnetic Resonance). TPP-3 was found to be a homogeneous heteropolysaccharide with an average molecular weight of 5.5 x 104 Da and composed of eight types of monosaccharides. The pro-angiogenic activities of TPP-3 were verified on HUVECs and VEGFR tyrosine kinase inhibitor II (VRI)-induced vascular defect zebrafish model. Furthermore, the underlying mechanism investigation showed that its pro-angiogenic activities were closely related with the activation of VEGF/PI3K/Akt signaling pathway.

引用

页码：2028 / 2040

页数：13

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