Structural determination and pro-angiogenic effect of polysaccharide from the pollen of Typha angustifolia L.

被引：9
|
作者：
Gao, Mingliang [1 ]
Lan, Jinshan [1 ]
Zha, Yuling [1 ]
Yao, Weifeng [1 ]
Bao, Beihua [1 ]
Shan, Mingqiu [1 ]
Zhang, Fang [1 ,2 ]
Zhou, Guisheng [1 ,2 ]
Yu, Sheng [1 ]
Cheng, Fangfang [1 ]
Cao, Yudan [1 ]
Yan, Hui [1 ,2 ]
Zhang, Li [1 ,2 ,3 ]
Chen, Peidong [1 ,2 ,3 ]
机构：
[1] Nanjing Univ Chinese Med, Sch Pharm, Nanjing 210023, Peoples R China
[2] Nanjing Univ Chinese Med, Jiangsu Collaborat Innovat Ctr Chinese Med Resourc, Sch Pharm, Nanjing 210023, Peoples R China
[3] Nanjing Univ Chinese Med, Xianlin Rd 138, Nanjing 210023, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2022年 / 222卷
基金：
中国国家自然科学基金;
关键词：
Polysaccharide; Typha angustifolia L; Structural determination; Pro-angiogenic; VEGF; PI3K; Akt signaling pathway; IN-VITRO; ANTIOXIDANT ACTIVITY; GROWTH-FACTOR; NITRIC-OXIDE; PURIFICATION; IDENTIFICATION; EXTRACTION; MANAGEMENT; STANDARDS; ACIDS;
D O I：
10.1016/j.ijbiomac.2022.10.002
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Four fractions of polysaccharides (TPP-1, TPP-2, TPP-3, and TPP-4) were isolated and purified from the pollen of Typha angustifolia L., and the structure of TPP-3 was furtherly determined by HPGPC (High Performance Gel Permeation Chromatography), monosaccharide composition analysis, methylation analysis and NMR (Nuclear Magnetic Resonance). TPP-3 was found to be a homogeneous heteropolysaccharide with an average molecular weight of 5.5 x 104 Da and composed of eight types of monosaccharides. The pro-angiogenic activities of TPP-3 were verified on HUVECs and VEGFR tyrosine kinase inhibitor II (VRI)-induced vascular defect zebrafish model. Furthermore, the underlying mechanism investigation showed that its pro-angiogenic activities were closely related with the activation of VEGF/PI3K/Akt signaling pathway.
引用
收藏
页码：2028 / 2040
页数：13
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