Anionic phospholipid-induced regulation of reactive oxygen species production by human cytochrome P450 2E1

被引：18

作者：

Cho, Eun Yi ^{[2
,3
]}

Yun, Chul-Ho ^{[2
,3
]}

Chae, Ho-Zoon ^{[2
,3
]}

Chae, Han-Jung ^{[4
,5
]}

Ahn, Taeho ^{[1
]}

机构：

[1] Chonnam Natl Univ, Dept Biochem, Coll Vet Med, Kwangju 500757, South Korea

[2] Chonnam Natl Univ, Sch Biol Sci & Technol, Kwangju 500757, South Korea

[3] Chonnam Natl Univ, Hormone Res Ctr, Kwangju 500757, South Korea

[4] Chonbuk Natl Univ, Sch Med, Dept Pharmacol, Jeonju 560182, South Korea

[5] Chonbuk Natl Univ, Sch Med, Inst Cardiovasc Res, Jeonju 560182, South Korea

来源：

FEBS LETTERS | 2008年 / 582卷 / 12期

基金：

新加坡国家研究基金会;

关键词：

cytochrome p450 2E1; anionic phospholipids; reactive oxygen species;

D O I：

10.1016/j.febslet.2008.04.048

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We suggest that the cytochrome P450 2E1 (CYP2E1)-induced formation of reactive oxygen species (ROS) can be regulated by anionic phospholipids and the presence of the N-terminal region of the enzyme. When the content of cardiolipin (CL) in membranes at the expense of phosphatidylcholine matrix was increased, the ROS produced by recombinant human CYP2E1 was decreased as a function of CL concentration. On the contrary, the N-terminally truncated CYP2E1 had a decreased effect on the lipid-induced reduction of ROS formation. These results suggest that specific phospholipids can regulate the function of CYP2E1 by interaction with the enzyme including the N-terminal region(s). (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：1771 / 1776

页数：6

共 37 条

[1] Membrane insertion of cytochrome P450 1A2 promoted by anionic phospholipids [J].