Isolation and characterization of a novel Rickettsia species (Rickettsia asembonensis sp nov.) obtained from cat fleas (Ctenocephalides felis)

被引：35
作者：
Maina, Alice N. [1 ]
Luce-Fedrow, Alison [1 ,2 ]
Omulo, Sylvia [3 ]
Hang, Jun [4 ]
Chan, Teik-Chye [1 ]
Ade, Fredrick [3 ]
Jima, Dereje D. [4 ]
Ogola, Eric [3 ]
Ge, Hong [1 ]
Breiman, Robert F. [5 ]
Njenga, Moses K. [3 ]
Richards, Allen L. [1 ]
机构：
[1] Naval Med Res Ctr, 503 Robert Grant Ave, Silver Spring, MD 20910 USA
[2] Shippensburg Univ, 1871 Old Main Dr, Shippensburg, PA 17257 USA
[3] Kenya Govt Med Res Ctr, Kisumu, Kenya
[4] Walter Reed Army Inst Res, Silver Spring, MD 20910 USA
[5] US Ctr Dis Control & Prevent, Nairobi, Kenya
来源：
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY | 2016年 / 66卷
关键词：
BARTONELLA; IDENTIFICATION; ASEMBOENSIS; DOGS; PHYLOGENY; ANAPLASMA; EHRLICHIA; ORGANISM; INSIGHT; GENES;
D O I：
10.1099/ijsem.0.001382
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A novel rickettsial agent, 'Candidatus Rickettsia asembonensis' strain NMRCii(T), was isolated from cat fleas, Ctenocephalides felis, from Kenya. Genotypic characterization of the new isolate based on sequence analysis of five rickettsial genes, rrs, gltA, ompA, ompB and sca4, indicated that this isolate clustered with Rickettsia felis URRWXCal2. The degree of nucleotide similarity demonstrated that isolate NMRCii(T) belongs within the genus Rickettsia and fulfils the criteria for classification as a representative of a novel species. The name Rickettsia asembonensis sp. nov. is proposed, with NMRCii(T) (=DSM 100172(T)=CDC CRIRC RAS001(T)=ATCC VR-1827(T)) as the type strain.
引用
收藏
页码：4512 / 4517
页数：6
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共 23 条
  • [1] Molecular Evidence of Different Rickettsia Species in Villeta, Colombia
    Faccini-Martinez, Alvaro A.
    Ramirez-Hernandez, Alejandro
    Forero-Becerra, Elkin
    Cortes-Vecino, Jesus A.
    Escandon, Patricia
    Rodas, Juan D.
    Palomar, Ana M.
    Portillo, Aranzazu
    Oteo, Jose A.
    Hidalgo, Marylin
    [J]. VECTOR-BORNE AND ZOONOTIC DISEASES, 2016, 16 (02) : 85 - 87
  • [2] Rickettsia, Ehrlichia, Anaplasma, and Bartonella in Ticks and Fleas from Dogs and Cats in Bangkok
    Foongladda, Suporn
    Inthawong, Dutsadee
    Kositanont, Uraiwan
    Gaywee, Jariyanart
    [J]. VECTOR-BORNE AND ZOONOTIC DISEASES, 2011, 11 (10) : 1335 - 1341
  • [3] Epidemiology of Spotted Fever Group and Typhus Group Rickettsial Infection in the Amazon Basin of Peru
    Forshey, Brett M.
    Stewart, Allison
    Morrison, Amy C.
    Galvez, Hugo
    Rocha, Claudio
    Astete, Helvio
    Eza, Dominique
    Chen, Hua-Wei
    Chao, Chien-Chung
    Montgomery, Joel M.
    Bentzel, David E.
    Ching, Wei-Mei
    Kochel, Tadeusz J.
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2010, 82 (04) : 683 - 690
  • [4] Gene sequence-based criteria for identification of new Rickettsia isolates and description of Rickettsia heilongjiangensis sp nov
    Fournier, PE
    Dumler, JS
    Greub, G
    Zhang, JZ
    Wu, YM
    Raoult, D
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (12) : 5456 - 5465
  • [5] Current Knowledge on Phylogeny and Taxonomy of Rickettsia spp.
    Fournier, Pierre-Edouard
    Raoult, Didier
    [J]. RICKETTSIOLOGY AND RICKETTSIAL DISEASES, 2009, 1166 : 1 - 11
  • [6] Polymerase Chain Reaction Detection of Rickettsia felis-Like Organism in Archaeopsylla erinacei (Siphonaptera: Pulicidae) From Bavaria, Germany
    Gilles, J.
    Silaghi, C.
    Just, F. T.
    Pradel, I.
    Pfister, K.
    [J]. JOURNAL OF MEDICAL ENTOMOLOGY, 2009, 46 (03) : 703 - 707
  • [7] Plasmids and Rickettsial Evolution: Insight from Rickettsia felis
    Gillespie, Joseph J.
    Beier, Magda S.
    Rahman, M. Sayeedur
    Ammerman, Nicole C.
    Shallom, Joshua M.
    Purkayastha, Anjan
    Sobral, Bruno S.
    Azad, Abdu F.
    [J]. PLOS ONE, 2007, 2 (03):
  • [8] A Rickettsia Genome Overrun by Mobile Genetic Elements Provides Insight into the Acquisition of Genes Characteristic of an Obligate Intracellular Lifestyle
    Gillespie, Joseph J.
    Joardar, Vinita
    Williams, Kelly P.
    Driscoll, Timothy
    Hostetler, Jessica B.
    Nordberg, Eric
    Shukla, Maulik
    Walenz, Brian
    Hill, Catherine A.
    Nene, Vishvanath M.
    Azad, Abdu F.
    Sobral, Bruno W.
    Caler, Elisabet
    [J]. JOURNAL OF BACTERIOLOGY, 2012, 194 (02) : 376 - 394
  • [9] Molecular detection of Rickettsia felis and Candidatus Rickettsia Asemboensis in Fleas from Human Habitats, Asembo, Kenya
    Jiang, Ju
    Maina, Alice N.
    Knobel, Darryn L.
    Cleaveland, Sarah
    Laudisoit, Anne
    Wamburu, Kabura
    Ogola, Eric
    Parola, Philippe
    Breiman, Robert F.
    Njenga, M. Kariuki
    Richards, Allen L.
    [J]. VECTOR-BORNE AND ZOONOTIC DISEASES, 2013, 13 (08) : 550 - 558
  • [10] Whole-Genome Sequence of "Candidatus Rickettsia asemboensis" Strain NMRCii, Isolated from Fleas of Western Kenya
    Jima, Dereje D.
    Luce-Fedrow, Alison
    Yang, Yu
    Maina, Alice N.
    Snesrud, Erik C.
    Otiang, Elkanah
    Njenga, Kariuki
    Jarman, Richard G.
    Richards, Allen L.
    Hang, Jun
    [J]. GENOME ANNOUNCEMENTS, 2015, 3 (02)
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