A human endothelial cell line is a convenient tool for exploring cell physiology and testing drugs and toxics. Several attempts have been made using SV40 to immortalize endothelial cells. We used human umbilical vein endothelial cells (HUVEC) transformed with a construct made of promoter of the vimentin gene and SV40 Tag. The proliferation of immortalized vascular endothelial cells (IVEC), as measured by [methyl-H-3]thymidine incorporation, was compared to that of HUVEC in the presence of endothelial cell growth factor and cytokines: tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) and interferon-gamma (IFN-gamma). Inhibition of [methyl-H-3]thymidine incorporation by IL-1 beta was lower than that observed with HUVEC, while TNF-alpha reduced the proliferation of IVEC and HUVEC to similar extents. Induction of intercellular adhesion molecule (ICAM-1), vascular cell adhesion molecule (VCAM-1) and E-selectin by TNF-alpha, measured by a radiometric technique, was similar in IVEC and HUVEC, while the induction of E-selectin by IL-1 beta on IVEC was limited and significantly different from that observed on HUVEC (p < 0.001). The number of I-125-IL-1 beta binding sites on IVEC is 3-fold less than on HUVEC and the IL-1 beta receptor number was reduced. Dexamethasone treatment of IVEC restored their reactivity to IL-1 beta and corrected the IL-1 beta binding and the receptor number. These results showed that the introduction of SV40 gene not only immortalized the cell but also altered IL-1 receptor expression. This alteration may be improved by addition of corticosteroids to the cell culture, which extends the possibility of using IVEC as a model of endothelial cells.
机构:
Univ N Carolina, Dept Med, Div Hematol & Oncol, McAllister Heart Inst, Chapel Hill, NC USAUniv N Carolina, Dept Med, Div Hematol & Oncol, McAllister Heart Inst, Chapel Hill, NC USA
Wang, Jian-Guo
Williams, Julie C.
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Univ N Carolina, Dept Med, Div Hematol & Oncol, McAllister Heart Inst, Chapel Hill, NC USAUniv N Carolina, Dept Med, Div Hematol & Oncol, McAllister Heart Inst, Chapel Hill, NC USA
Williams, Julie C.
Davis, Beckley K.
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h-index: 0
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Univ N Carolina, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USAUniv N Carolina, Dept Med, Div Hematol & Oncol, McAllister Heart Inst, Chapel Hill, NC USA
Davis, Beckley K.
Jacobson, Ken
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h-index: 0
机构:
Univ N Carolina, Dept Cell & Dev Biol, Chapel Hill, NC USAUniv N Carolina, Dept Med, Div Hematol & Oncol, McAllister Heart Inst, Chapel Hill, NC USA
Jacobson, Ken
Doerschuk, Claire M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ N Carolina, Dept Med, Ctr Airways Dis, Chapel Hill, NC USAUniv N Carolina, Dept Med, Div Hematol & Oncol, McAllister Heart Inst, Chapel Hill, NC USA
Doerschuk, Claire M.
Ting, Jenny P. -Y.
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h-index: 0
机构:
Univ N Carolina, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USAUniv N Carolina, Dept Med, Div Hematol & Oncol, McAllister Heart Inst, Chapel Hill, NC USA
Ting, Jenny P. -Y.
Mackman, Nigel
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h-index: 0
机构:
Univ N Carolina, Dept Med, Div Hematol & Oncol, McAllister Heart Inst, Chapel Hill, NC USAUniv N Carolina, Dept Med, Div Hematol & Oncol, McAllister Heart Inst, Chapel Hill, NC USA
机构:
La Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USALa Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USA
Anquetil, Florence
Sabouri, Somayeh
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h-index: 0
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La Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USALa Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USA
Sabouri, Somayeh
Thivolet, Charles
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h-index: 0
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La Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USALa Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USA
Thivolet, Charles
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Rodriguez-Calvo, Teresa
Zapardiel-Gonzalo, Jose
论文数: 0引用数: 0
h-index: 0
机构:
La Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USALa Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USA
Zapardiel-Gonzalo, Jose
Amirian, Natalie
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机构:
La Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USALa Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USA
Amirian, Natalie
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Schneider, Darius
Castillo, Ericka
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h-index: 0
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La Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USALa Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USA
Castillo, Ericka
Lajevardi, Yasaman
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h-index: 0
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La Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USALa Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USA
Lajevardi, Yasaman
von Herrath, Matthias G.
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h-index: 0
机构:
La Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USA
Novo Nordisk Diabet Res & Dev Ctr, Seattle, WA USALa Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USA
机构:
Scripps Res Inst, Dept Immunol & Microbial Sci, La Jolla, CA 92037 USAScripps Res Inst, Dept Immunol & Microbial Sci, La Jolla, CA 92037 USA
Lu, Jiawei
Wu, Xiurong
论文数: 0引用数: 0
h-index: 0
机构:
Xiamen Univ, Sch Life Sci, State Key Lab Stress Cell Biol, Xiamen 361005, Fujian, Peoples R ChinaScripps Res Inst, Dept Immunol & Microbial Sci, La Jolla, CA 92037 USA
Wu, Xiurong
Hong, Mao
论文数: 0引用数: 0
h-index: 0
机构:
Xiamen Univ, Sch Life Sci, State Key Lab Stress Cell Biol, Xiamen 361005, Fujian, Peoples R ChinaScripps Res Inst, Dept Immunol & Microbial Sci, La Jolla, CA 92037 USA
Hong, Mao
Tobias, Peter
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Immunol & Microbial Sci, La Jolla, CA 92037 USAScripps Res Inst, Dept Immunol & Microbial Sci, La Jolla, CA 92037 USA
Tobias, Peter
Han, Jiahuai
论文数: 0引用数: 0
h-index: 0
机构:
Xiamen Univ, Sch Life Sci, State Key Lab Stress Cell Biol, Xiamen 361005, Fujian, Peoples R ChinaScripps Res Inst, Dept Immunol & Microbial Sci, La Jolla, CA 92037 USA
机构:
Univ N Carolina, Dept Med, Div Hematol & Oncol, McAllister Heart Inst, Chapel Hill, NC USAUniv N Carolina, Dept Med, Div Hematol & Oncol, McAllister Heart Inst, Chapel Hill, NC USA
Wang, Jian-Guo
Williams, Julie C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ N Carolina, Dept Med, Div Hematol & Oncol, McAllister Heart Inst, Chapel Hill, NC USAUniv N Carolina, Dept Med, Div Hematol & Oncol, McAllister Heart Inst, Chapel Hill, NC USA
Williams, Julie C.
Davis, Beckley K.
论文数: 0引用数: 0
h-index: 0
机构:
Univ N Carolina, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USAUniv N Carolina, Dept Med, Div Hematol & Oncol, McAllister Heart Inst, Chapel Hill, NC USA
Davis, Beckley K.
Jacobson, Ken
论文数: 0引用数: 0
h-index: 0
机构:
Univ N Carolina, Dept Cell & Dev Biol, Chapel Hill, NC USAUniv N Carolina, Dept Med, Div Hematol & Oncol, McAllister Heart Inst, Chapel Hill, NC USA
Jacobson, Ken
Doerschuk, Claire M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ N Carolina, Dept Med, Ctr Airways Dis, Chapel Hill, NC USAUniv N Carolina, Dept Med, Div Hematol & Oncol, McAllister Heart Inst, Chapel Hill, NC USA
Doerschuk, Claire M.
Ting, Jenny P. -Y.
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h-index: 0
机构:
Univ N Carolina, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USAUniv N Carolina, Dept Med, Div Hematol & Oncol, McAllister Heart Inst, Chapel Hill, NC USA
Ting, Jenny P. -Y.
Mackman, Nigel
论文数: 0引用数: 0
h-index: 0
机构:
Univ N Carolina, Dept Med, Div Hematol & Oncol, McAllister Heart Inst, Chapel Hill, NC USAUniv N Carolina, Dept Med, Div Hematol & Oncol, McAllister Heart Inst, Chapel Hill, NC USA
机构:
La Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USALa Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USA
Anquetil, Florence
Sabouri, Somayeh
论文数: 0引用数: 0
h-index: 0
机构:
La Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USALa Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USA
Sabouri, Somayeh
Thivolet, Charles
论文数: 0引用数: 0
h-index: 0
机构:
La Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USALa Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USA
Thivolet, Charles
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机构:
Rodriguez-Calvo, Teresa
Zapardiel-Gonzalo, Jose
论文数: 0引用数: 0
h-index: 0
机构:
La Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USALa Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USA
Zapardiel-Gonzalo, Jose
Amirian, Natalie
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h-index: 0
机构:
La Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USALa Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USA
Amirian, Natalie
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机构:
Schneider, Darius
Castillo, Ericka
论文数: 0引用数: 0
h-index: 0
机构:
La Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USALa Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USA
Castillo, Ericka
Lajevardi, Yasaman
论文数: 0引用数: 0
h-index: 0
机构:
La Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USALa Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USA
Lajevardi, Yasaman
von Herrath, Matthias G.
论文数: 0引用数: 0
h-index: 0
机构:
La Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USA
Novo Nordisk Diabet Res & Dev Ctr, Seattle, WA USALa Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA USA
机构:
Scripps Res Inst, Dept Immunol & Microbial Sci, La Jolla, CA 92037 USAScripps Res Inst, Dept Immunol & Microbial Sci, La Jolla, CA 92037 USA
Lu, Jiawei
Wu, Xiurong
论文数: 0引用数: 0
h-index: 0
机构:
Xiamen Univ, Sch Life Sci, State Key Lab Stress Cell Biol, Xiamen 361005, Fujian, Peoples R ChinaScripps Res Inst, Dept Immunol & Microbial Sci, La Jolla, CA 92037 USA
Wu, Xiurong
Hong, Mao
论文数: 0引用数: 0
h-index: 0
机构:
Xiamen Univ, Sch Life Sci, State Key Lab Stress Cell Biol, Xiamen 361005, Fujian, Peoples R ChinaScripps Res Inst, Dept Immunol & Microbial Sci, La Jolla, CA 92037 USA
Hong, Mao
Tobias, Peter
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Immunol & Microbial Sci, La Jolla, CA 92037 USAScripps Res Inst, Dept Immunol & Microbial Sci, La Jolla, CA 92037 USA
Tobias, Peter
Han, Jiahuai
论文数: 0引用数: 0
h-index: 0
机构:
Xiamen Univ, Sch Life Sci, State Key Lab Stress Cell Biol, Xiamen 361005, Fujian, Peoples R ChinaScripps Res Inst, Dept Immunol & Microbial Sci, La Jolla, CA 92037 USA