Biological abatement of cellulase inhibitors

被引：37

作者：

Cao, Guangli ^{[1
,3
]}

Ximenes, Eduardo ^{[1
]}

Nichols, Nancy N. ^{[2
]}

Zhang, Leyu ^{[1
]}

Ladisch, Michael ^{[1
]}

机构：

[1] Purdue Univ, Renewable Resources Engn Lab, W Lafayette, IN 47907 USA

[2] USDA ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA

[3] Harbin Inst Technol, Dept Life Sci & Technol, Harbin 150001, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2013年 / 146卷

关键词：

Bio-abatement; Inhibitors; Biomass liquor; Enzymatic hydrolysis; ENZYMATIC-HYDROLYSIS; FERMENTATION INHIBITORS; LIGNOCELLULOSIC BIOMASS; DETOXIFICATION; ETHANOL; PRETREATMENT; FERMENTABILITY; DIGESTIBILITY; RECALCITRANCE; BIOCONVERSION;

D O I：

10.1016/j.biortech.2013.07.112

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Removal of enzyme inhibitors released during lignocellulose pretreatment is essential for economically feasible biofuel production. We tested bio-abatement to mitigate enzyme inhibitor effects observed in corn stover liquors after pretreatment with either dilute acid or liquid hot water at 10% (w/v) solids. Bio-abatement of liquors was followed by enzymatic hydrolysis of cellulose. To distinguish between inhibitor effects on enzymes and recalcitrance of the substrate, pretreated corn stover solids were removed and replaced with 1% (w/v) Solka Floc. Cellulose conversion in the presence of bio-abated liquors from dilute acid pretreatment was 8.6% (0.1x enzyme) and 16% (1x enzyme) higher than control (non-abated) samples. In the presence of bio-abated liquor from liquid hot water pretreated corn stover, 10% (0.1x enzyme) and 13% (1x enzyme) higher cellulose conversion was obtained compared to control. Bio-abatement yielded improved enzyme hydrolysis in the same range as that obtained using a chemical (overliming) method for mitigating inhibitors. (c) 2013 Elsevier Ltd. All rights reserved.

引用

页码：604 / 610

页数：7

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