Biodegradation of chlorpyrifos and its hydrolyzing metabolite 3,5,6-trichloro-2-pyridinol by Sphingobacterium sp JAS3

被引：72

作者：

Abraham, Jayanthi ^{[1
]}

Silambarasan, Sivagnanam ^{[1
]}

机构：

[1] VIT Univ, Sch Biosci & Technol, Microbial Biotechnol Lab, Vellore 632014, Tamil Nadu, India

来源：

PROCESS BIOCHEMISTRY | 2013年 / 48卷 / 10期

关键词：

Biodegradation; Chlorpyrifos; 3,5,6-Trichloro-2-pyridinol; Sphingobacterium sp JAS3; DEGRADING BACTERIUM; METHYL PARATHION; STRAIN; CLONING; DEGRADATION; BIOREMEDIATION; PESTICIDES; GENE;

D O I：

10.1016/j.procbio.2013.06.034

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Biodegradation of chlorpyrifos and its metabolite 3,5,6-trichloro-2-pyridinol (TCP) were studied in aqueous medium and in soil with a novel bacterial strain JAS3. The molecular characterization based on 16S rRNA sequence analysis revealed the strain JAS3 as Sphingobacterium sp. The strain JAS3 was able to grow in minimal salt medium (MSM) supplemented with 300 mg l(-1) of chlorpyrifos as sole carbon source. The degradation of chlorpyrifos and its primary metabolite TCP were examined by HPLC. After 5 d, Sphingobacterium sp. JAS3 degraded chlorpyrifos and its metabolite TCP to benzene, 1,3-bis(1,1-dimethylethyl) was analyzed by GCMS. Degradation of chlorpyrifos and TCP in soil with and without addition of nutrients was also studied. The ability to degrade chlorpyrifos makes this strain a useful candidate for remediation of pesticide contaminated sites. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：1559 / 1564

页数：6

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