A cationic sub-micron emulsion (MF59/DOTAP) is an effective delivery system for DNA vaccines

被引：55
|
作者：
Ott, G [1 ]
Singh, M [1 ]
Kazzaz, J [1 ]
Briones, M [1 ]
Soenawan, E [1 ]
Ugozzoli, M [1 ]
O'Hagan, DT [1 ]
机构：
[1] Chiron Corp, Emeryville, CA 94608 USA
来源：
JOURNAL OF CONTROLLED RELEASE | 2002年 / 79卷 / 1-3期
关键词：
cationic emulsion; DNA delivery; vaccine adjuvant;
D O I：
10.1016/S0168-3659(01)00545-4
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A novel cationic emulsion was developed to adsorb plasmid DNA and improve intracellular delivery of plasmid DNA upon immunization. The emulsion developed, had a highly uniform particle and charge distribution. Based on observations with cationic microparticles, the cationic emulsion was evaluated in vivo in mice and rabbits with a model HIV-1 pCMVp55 gag DNA. In both these species, the cationic emulsion engendered higher antibody responses than those obtained with naked DNA. The cationic emulsion also maintained the cellular responses seen with naked DNA at the same doses. (C) 2002 Elsevier Science B.V. All rights reserved.
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页码：1 / 5
页数：5
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