Substrate profiling of Zika virus NS2B-NS3 protease

被引：45

作者：

Gruba, Natalia ^{[1
]}

Martinez, Jose Ignacio Rodriguez ^{[2
]}

Grzywa, Renata ^{[3
]}

Wysocka, Magdalena ^{[1
]}

Skorenski, Marcin ^{[3
]}

Burmistrz, Michal ^{[2
,4
]}

Lecka, Maria ^{[3
]}

Lesner, Adam ^{[1
]}

Sienczyk, Marcin ^{[3
]}

Pyrc, Krzysztof ^{[2
,4
]}

机构：

[1] Univ Gdansk, Fac Chem, Dept Biochem, Wita Stwosza 63, PL-80952 Gdansk, Poland

[2] Jagiellonian Univ, Fac Biochem Biophys & Biotechnol, Dept Microbiol, Krakow, Poland

[3] Wroclaw Univ Sci & Technol, Fac Chem, Div Med Chem & Microbiol, Wybrzeze Wyspianskiego 27, PL-50370 Wroclaw, Poland

[4] Jagiellonian Univ, Malopolska Ctr Biotechnol, Krakow, Poland

来源：

FEBS LETTERS | 2016年 / 590卷 / 20期

关键词：

combinatorial chemistry; NS2B-NS3; protease; substrate library; substrate mapping; Zika virus; DENGUE VIRUS; NS3; PROTEASE; NS2B/NS3; SERINE-PROTEASE; LIGAND DOCKING; AMINO-ACIDS; DRUG DESIGN; SPECIFICITY; INHIBITORS; PROSPECTS;

D O I：

10.1002/1873-3468.12443

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Zika virus (ZIKV), isolated from macaques in Uganda in 1947, was not considered to be a dangerous human pathogen. However, this view has recently changed as ZIKV infections are now associated with serious pathological disorders including microcephaly and Guillain-Barre syndrome. Similar to other viruses in the Flaviviridae family, ZIKV expresses the serine protease NS3 which is responsible for viral protein processing and replication. Herein, we report the expression of an active NS3(pro) domain fused with the NS2B cofactor (NS2B(LN)NS3(pro)) in a prokaryotic expression system and profile its specificity for synthesized FRET-type substrate libraries. Our findings pave way for screening potential intracellular substrates of NS3 and for developing specific inhibitors of this ZIKV protease.

引用

页码：3459 / 3468

页数：10

共 42 条

[1] Structural evidence for regulation and specificity of flaviviral proteases and evolution of the Flaviviridae fold
Aleshin, Alexander E.
Shiryaev, Sergey A.
Strongin, Alex Y.
Liddington, Robert C.
[J]. PROTEIN SCIENCE, 2007, 16 (05) : 795 - 806
[2] A P2 and P3 substrate specificity comparison between the Murray Valley encephalitis and West Nile virus NS2B/NS3 protease using C-terminal agmatine dipeptides
Ang, Melgious Jin Yan
Li, Zhitao
Lim, Huichang Annie
Ng, Fui Mee
Then, Siew Wen
Wee, John Liang Kuan
Joy, Joma
Hill, Jeffrey
Chia, C. S. Brian
[J]. PEPTIDES, 2014, 52 : 49 - 52
[3] Crystal Structure of a Novel Conformational State of the Flavivirus NS3 Protein: Implications for Polyprotein Processing and Viral Replication
Assenberg, Rene
Mastrangelo, Eloise
Walter, Thomas S.
Verma, Anil
Milani, Mario
Owens, Raymond J.
Stuart, David I.
Grimes, Jonathan M.
Mancini, Erika J.
[J]. JOURNAL OF VIROLOGY, 2009, 83 (24) : 12895 - 12906
[4] BEARCROFT W. G. C., 1956, TRANS ROY SOC TROP MED AND HYG, V50, P442, DOI 10.1016/0035-9203(56)90090-6
[5] The Medicinal Chemistry of Dengue Virus
Behnam, Mira A. M.
Nitsche, Christoph
Boldescu, Veaceslav
Klein, Christian D.
[J]. JOURNAL OF MEDICINAL CHEMISTRY, 2016, 59 (12) : 5622 - 5649
[6] Zika Virus Outbreak, Bahia, Brazil
Campos, Gubio S.
Bandeira, Antonio C.
Sardi, Silvia I.
[J]. EMERGING INFECTIOUS DISEASES, 2015, 21 (10) : 1885 - 1886
[7] Guillain-Barre Syndrome outbreak associated with Zika virus infection in French Polynesia: a case-control study
Cao-Lormeau, Van-Mai
Blake, Alexandre
Mons, Sandrine
Lastere, Stephane
Roche, Claudine
Vanhomwegen, Jessica
Dub, Timothee
Baudouin, Laure
Teissier, Anita
Larre, Philippe
Vial, Anne-Laure
Decam, Christophe
Choumet, Valerie
Halstead, Susan K.
Willison, Hugh J.
Musset, Lucile
Manuguerra, Jean-Claude
Despres, Philippe
Fournier, Emmanuel
Mallet, Henri-Pierre
Musso, Didier
Fontanet, Arnaud
Neil, Jean
Ghawche, Frederic
[J]. LANCET, 2016, 387 (10027) : 1531 - 1539
[8] West Nile Virus NS2B/NS3 Protease As An Antiviral Target
Chappell, K. J.
Stoermer, M. J.
Fairlie, D. P.
Young, P. R.
[J]. CURRENT MEDICINAL CHEMISTRY, 2008, 15 (27) : 2771 - 2784
[9] The Brazilian Zika virus strain causes birth defects in experimental models
Cugola, Fernanda R.
Fernandes, Isabella R.
Russo, Fabiele B.
Freitas, Beatriz C.
Dias, Joao L. M.
Guimaraes, Katia P.
Benazzato, Cecilia
Almeida, Nathalia
Pignatari, Graciela C.
Romero, Sarah
Polonio, Carolina M.
Cunha, Isabela
Freitas, Carla L.
Brandao, Wesley N.
Rossato, Cristiano
Andrade, David G.
Faria, Daniele de P.
Garcez, Alexandre T.
Buchpigel, Carlos A.
Braconi, Carla T.
Mendes, Erica
Sall, Amadou A.
Zanotto, Paolo M. de A.
Peron, Jean Pierre S.
Muotri, Alysson R.
Beltrao-Braga, Patricia C. B.
[J]. NATURE, 2016, 534 (7606) : 267 - +
[10] ZIKA VIRUS .1. ISOLATIONS AND SEROLOGICAL SPECIFICITY
DICK, GWA
KITCHEN, SF
HADDOW, AJ
[J]. TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1952, 46 (05) : 509 - 520

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