Antioxidant Profile of Trifolium pratense L.

被引:42
作者
Kaurinovic, Biljana [1 ]
Popovic, Mira [1 ]
Vlaisavljevic, Sanja [1 ]
Schwartsova, Heidy [2 ]
Vojinovic-Miloradov, Mirjana [3 ]
机构
[1] Fac Sci, Dept Chem Biochem & Environm Protect, Novi Sad 21000, Serbia
[2] Middle European High Sch, Skalica 90901, Slovakia
[3] Fac Tech Sci, Environm Protect Dept, Novi Sad 21000, Serbia
关键词
Trifolium pratense; antioxidant activity; flavonoids; biochemical parameters; RED-CLOVER; XANTHINE-OXIDASE; ISOFLAVONES; FLAVONOIDS; INHIBITION; BIOCHEMISTRY;
D O I
10.3390/molecules170911156
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In order to examine the antioxidant properties of five different extracts of Trifolium pratense L. (Leguminosae) leaves, various assays which measure free radical scavenging ability were carried out: 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, superoxide anion and nitric oxide radical scavenger capacity tests and lipid peroxidation assay. In all of the tests, only the H2O and (to some extent) the EtOAc extracts showed a potent antioxidant effect compared with BHT and BHA, well-known synthetic antioxidants. In addition, in vivo experiments were conducted with antioxidant systems (activities of GSHPx, GSHR, Px, CAT, XOD, GSH content and intensity of LPx) in liver homogenate and blood of mice after their treatment with extracts of T. pratense leaves, or in combination with CCl4. Besides, in the extracts examined the total phenolic and flavonoid amounts were also determined, together with presence of the selected flavonoids: quercetin, luteolin, apigenin, naringenin and kaempferol, which were studied using a HPLC-DAD technique. HPLC-DAD analysis showed a noticeable content of natural products according to which the examined Trifolium pratense species could well be regarded as a promising new source of bioactive natural compounds, which can be used both as a food supplement and a remedy.
引用
收藏
页码:11156 / 11172
页数:17
相关论文
共 59 条
[1]   CHELATING AND FREE-RADICAL SCAVENGING MECHANISMS OF INHIBITORY-ACTION OF RUTIN AND QUERCETIN IN LIPID-PEROXIDATION [J].
AFANASEV, IB ;
DOROZHKO, AI ;
BRODSKII, AV ;
KOSTYUK, VA ;
POTAPOVITCH, AI .
BIOCHEMICAL PHARMACOLOGY, 1989, 38 (11) :1763-1769
[2]   Structure-activity relationships for antioxidant activities of a series of flavonoids in a liposomal system [J].
Arora, A ;
Nair, MG ;
Strasburg, GM .
FREE RADICAL BIOLOGY AND MEDICINE, 1998, 24 (09) :1355-1363
[3]   Antioxidant and hepatoprotective effect of Acanthus ilicifolius [J].
Babu, BH ;
Shylesh, BS ;
Padikkala, J .
FITOTERAPIA, 2001, 72 (03) :272-277
[4]  
Beers R FJ., 1950, J Biol Chem, V195, P133
[5]  
Bergmayer U H., 1970, Methoden der enzymatischen Analyse
[6]   ANTIOXIDANT DETERMINATIONS BY THE USE OF A STABLE FREE RADICAL [J].
BLOIS, MS .
NATURE, 1958, 181 (4617) :1199-1200
[7]  
BORS W, 1990, METHOD ENZYMOL, V186, P343
[8]  
Bown D., 1995, ENCY HERBS THEIR USE
[9]  
Buege A.J., 1988, METHOD ENZYMOL, P302
[10]   Effect of dietary genistein on antioxidant enzyme activities in SENCAR mice [J].
Cai, QY ;
Wei, HC .
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 1996, 25 (01) :1-7