Extraction, characterization and anti-inflammatory activities of an inulin-type fructan from Codonopsis pilosula

被引：72

作者：

Meng, Yan ^{[1
]}

Xu, Yujie ^{[1
]}

Chang, Cong ^{[1
]}

Qiu, Zhenpeng ^{[1
]}

Hu, Junjie ^{[1
]}

Wu, Yong ^{[1
]}

Zhang, Baohui ^{[1
]}

Zheng, Guohua ^{[2
]}

机构：

[1] Hubei Univ Chinese Med, Coll Pharm, Wuhan 430065, Peoples R China

[2] Hubei Univ Chinese Med, Key Lab Chinese Med Resource & Compound Prescript, Wuhan 430065, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2020年 / 163卷

基金：

中国国家自然科学基金;

关键词：

Polysaccharides; Structure characterization; Anti-inflammatory; STRUCTURAL-CHARACTERIZATION; PECTIC POLYSACCHARIDE; LIGHT-SCATTERING; INDUCED COLITIS; IN-VITRO; ROOTS; CONFORMATION; FLEXIBILITY; MACROPHAGES; ACTIVATION;

D O I：

10.1016/j.ijbiomac.2020.09.117

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A homogenous polysaccharide from Codonopsis pilosula (CPP) was isolated and identified to be an inulin-type fructan, coded as CPP1-2-1. The polysaccharide exhibited anti-inflammatory effect against lipopolysaccharide (LPS) induced RAW264.7 cells in vitro and dextran sodium sulfate (DSS)-induced colitis mice in vivo. Moreover, the expression of cytokines was further detected by qPCR, and the results showed that the polysaccharides can reduce the expression of inflammatory factors such as TLR4, NF-kappa B, TNF-alpha and IL-6 in the cells, indicating the anti-inflammatory activities may be achieved by inhibiting the activation of TLR4/NF-kappa B pathway. (C) 2020 Elsevier B.V. All rights reserved.

引用

页码：1677 / 1686

页数：10

共 51 条

[1] Immune recognition -: A new receptor for β-glucans [J].

Brown, GD ;

Gordon, S .

NATURE, 2001, 413 (6851) :36-37

[2] Overview of the marine Roseobacter lineage [J].

Buchan, A ;

González, JM ;

Moran, MA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (10) :5665-5677

[3] NEW FEATURES OF PLANT-FRUCTAN STRUCTURE REVEALED BY METHYLATION ANALYSIS AND C-13 NMR-SPECTROSCOPY [J].

CARPITA, NC ;

HOUSLEY, TL ;

HENDRIX, JE .

CARBOHYDRATE RESEARCH, 1991, 217 :127-136

[4] Isolation, structural elucidation and immunomodulatory activity of fructans from aged garlic extract [J].

Chandrashekar, Puthanapura M. ;

Prashanth, Keelara V. Harish ;

Venkatesh, Yeldur P. .

PHYTOCHEMISTRY, 2011, 72 (2-3) :255-264

[5]

Chen Y., 2016, INT J TRADITIONAL CH, V38, P855

[6] Per-O-methylation reaction for structural analysis of carbohydrates by mass spectrometry [J].

Ciucanu, Ionel .

ANALYTICA CHIMICA ACTA, 2006, 576 (02) :147-155

[7] A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].

DUBOIS, M ;

GILLES, K ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

NATURE, 1951, 168 (4265) :167-167

[8] MEASUREMENT OF URONIC-ACIDS WITHOUT INTERFERENCE FROM NEUTRAL SUGARS [J].

FILISETTICOZZI, TMCC ;

CARPITA, NC .

ANALYTICAL BIOCHEMISTRY, 1991, 197 (01) :157-162

[9] Characterization and prebiotic activity in vitro of inulin-type fructan from Codonopsis pilosula roots [J].

Fu, Yu-Ping ;

Li, Li-Xia ;

Zhang, Bing-Zhao ;

Paulsen, Berit Smestad ;

Yin, Zhong-Qiong ;

Huang, Chao ;

Feng, Bin ;

Chen, Xing-Fu ;

Jia, Ren-Rong ;

Song, Xu ;

Ni, Xue-Qin ;

Jing, Bo ;

Wu, Fa-ming ;

Zou, Yuan-feng .

CARBOHYDRATE POLYMERS, 2018, 193 :212-220

[10] CLASSIFICATION OF GRASS FRUCTANS BY C-13 NMR-SPECTROSCOPY [J].

HAMMER, H ;

MORGENLIE, S .

ACTA CHEMICA SCANDINAVICA, 1990, 44 (02) :158-160

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