Suppressive effects of saponin-enriched extracts from quinoa on 3T3-L1 adipocyte differentiation

被引:41
作者
Yao, Yang [1 ]
Zhu, Yingying [1 ,2 ]
Gao, Yue [1 ,3 ]
Shi, Zhenxing [1 ]
Hu, Yibo [1 ]
Ren, Guixing [1 ]
机构
[1] Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
[2] Univ Liege, Lab Biophys & Formulat Engn, Gembloux Agrobio Tech, B-5030 Gembloux, Belgium
[3] Qilu Univ Technol, Jinan 250353, Shandong, Peoples R China
关键词
ACTIVATED RECEPTOR-GAMMA; PPAR-GAMMA; CHENOPODIUM-QUINOA; GLUCOSE-TRANSPORTER-4; TRANSLOCATION; AMPK PATHWAY; C/EBP-ALPHA; ADIPOGENESIS; CELLS; PREADIPOCYTES; EXPRESSION;
D O I
10.1039/c5fo00716j
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This study was performed to investigate the effect of quinoa saponins (QS) on the differentiation of 3T3-L1 preadipocytes. QS inhibited triglyceride (TG) accumulation in the mature adipocytes, evidenced by oilred O staining and intracellular quantification. Real time-PCR analysis and western blot analysis showed that QS significantly down-regulated the mRNA and protein expression of key adipogenic transcription factors, peroxisome proliferator-activated receptor gamma (PPAR gamma), and CCAAT/enhancer-binding protein alpha (C/EBP alpha), however, they had no significant effect on CCAAT/enhancer-binding protein beta (C/EBP beta) and CCAAT/enhancer-binding protein delta (C/EBPd) which are the upstream regulators for adipogenesis compared with mature adipocytes. QS also reduced mRNA and protein expression of sterol regulatory element-binding protein-1c (SREBP-1c) related to the late stage of adipogenesis. Furthermore, lipoprotein lipase (LPL), adipocyte protein 2 (aP2) and glucose transporter 4 (Glut4), as adipocyte specific genes, were decreased in mature adipocytes by QS treatment. These findings indicate that QS are capable of suppressing adipogenesis and therefore they seem to be natural bioactive factors effective in adipose tissue mass modulation.
引用
收藏
页码:3282 / 3290
页数:9
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