Rapid-Antigen Detection Tests for Group A Streptococcal Pharyngitis: Revisiting False-Positive Results Using Polymerase Chain Reaction Testing

被引：25

作者：

Cohen, Jeremie F. ^{[1
,2
]}

Cohen, Robert ^{[3
,4
]}

Bidet, Philippe ^{[5
]}

Levy, Corinne ^{[3
,6
]}

Deberdt, Patrice ^{[3
]}

d'Humieres, Camille ^{[5
]}

Liguori, Sandrine ^{[5
]}

Corrard, Francois ^{[3
]}

Thollot, Franck ^{[7
]}

Mariani-Kurkdjian, Patricia ^{[5
]}

Chalumeau, Martin ^{[1
,2
]}

Bingen, Edouard ^{[5
]}

机构：

[1] INSERM, UMR S953, Epidemiol Res Unit Perinatal Hlth & Womens & Chil, F-75014 Paris, France

[2] Univ Paris 05, Sorbonne Paris Cite, Necker Enfants Malad Hosp, AP HP,Dept Pediat, Paris, France

[3] Assoc Clin & Therapeut Val de Marne ACTIV, St Maur Des Fosses, France

[4] Ctr Hosp Intercommunal Creteil, Dept Microbiol, Creteil, France

[5] Univ Paris Diderot, Sorbonne Paris Cite, Robert Debre Hosp, AP HP,Dept Microbiol, Paris, France

[6] Ctr Hosp Intercommunal Creteil, Ctr Rech Clin, Creteil, France

[7] Assoc Francaise Pediat Ambulatoire, Esseys Les Nancy, France

来源：

JOURNAL OF PEDIATRICS | 2013年 / 162卷 / 06期

关键词：

PYOGENES;

D O I：

10.1016/j.jpeds.2013.01.050

中图分类号：

R72 [儿科学];

学科分类号：

100202 ;

摘要：

We investigated mechanisms of the false-positive test results on rapid-antigen detection test (RADT) for group A Streptococcal (GAS) pharyngitis. Most RADT false-positives (76%) were associated with polymerase chain reaction-positive GAS results, suggesting that RADT specificity could be considered close to 100%. Finding that 61% of GAS culture-negative but RADT-positive cases were positive on both GAS polymerase chain reaction and Staphylococcus aureus testing, we posit bacterial inhibition as causative.

引用

页码：1282 / U258

页数：4

共 10 条

[1] Identification of group A streptococcal emm types commonly associated with invasive infections and antimicrobial resistance by the use of multiplex PCR and high-resolution melting analysis
Bidet, P.
Liguori, S.
Plainvert, C.
Bonacorsi, S.
Courroux, C.
d'Humieres, C.
Poyart, C.
Efstratiou, A.
Bingen, E.
[J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2012, 31 (10) : 2817 - 2826
[2] Bacterial interference
Brook, I
[J]. CRITICAL REVIEWS IN MICROBIOLOGY, 1999, 25 (03) : 155 - 172
[3] Spectrum and Inoculum Size Effect of a Rapid Antigen Detection Test for Group A Streptococcus in Children with Pharyngitis
Cohen, Jeremie F.
Chalumeau, Martin
Levy, Corinne
Bidet, Philippe
Thollot, Franck
Wollner, Alain
Bingen, Edouard
Cohen, Robert
[J]. PLOS ONE, 2012, 7 (06):
[4] GERBER MA, 1987, PEDIATR INFECT DIS J, V6, P489
[5] Invasive group a streptococcal disease in Taiwan is not associated with the presence of streptococcal pyrogenic exotoxin genes
Hsueh, PR
Wu, JJ
Tsai, PJ
Liu, JW
Chuang, YC
Luh, KT
[J]. CLINICAL INFECTIOUS DISEASES, 1998, 26 (03) : 584 - 589
[6] False-positive rapid antigen detection test results: Reduced specificity in the absence of group A streptococci in the upper respiratory tract
Johnson, DR
Kaplan, EL
[J]. JOURNAL OF INFECTIOUS DISEASES, 2001, 183 (07) : 1135 - 1137
[7] Polymerase chain reaction for Streptococcus pyogenes used to evaluate an optical immunoassay for the detection of group A streptococci in children with pharyngitis
Kaltwasser, G
Diego, J
WelbySellenriek, PL
Ferrett, R
Caparon, M
Storch, GA
[J]. PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1997, 16 (08) : 748 - 753
[8] False positive detection of group A Streptococcus antigen resulting from cross-reacting Streptococcus intermedius (Streptococcus milleri group)
Rubin, LG
Kahn, RA
Vellozzi, EM
Isenberg, HD
[J]. PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1996, 15 (08) : 715 - 717
[9] Apparent false positive detection of group A Streptococcus antigen resulting from pharyngeal infection with a nonhemolytic Streptococcus pyogenes
Rubin, LG
Mirkin, GS
[J]. PEDIATRIC INFECTIOUS DISEASE JOURNAL, 2000, 19 (07) : 672 - 674
[10] False positive Strep A antigen test
Schroeder, S
Procop, GW
[J]. PEDIATRIC INFECTIOUS DISEASE JOURNAL, 2000, 19 (11) : 1114 - 1115

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