Complexation of HIV derived peptides with carbosilane dendrimers

被引:39
作者
Ionov, Maksim [1 ]
Ciepluch, Karol [1 ]
Klajnert, Barbara [1 ]
Glinska, Slawa [2 ]
Gomez-Ramirez, Rafael [3 ]
Javier de la Mata, Francisco [3 ]
Angeles Munoz-Fernandez, Maria [4 ]
Bryszewska, Maria [1 ]
机构
[1] Univ Lodz, Dept Gen Biophys, PL-90236 Lodz, Poland
[2] Univ Lodz, Lab Electron Microscopy, Fac Biol & Environm Protect, PL-90236 Lodz, Poland
[3] Univ Alcala de Henares, CIBER BBN Alcala de Henares, Dept Quim Inorgan, Alcala De Henares 99775, Spain
[4] Gen Hosp Univ Gregorio Maranon, CIBER BBN, Lab InmunoBiol Mol, Madrid, Spain
关键词
Carbosilane dendrimers; HIV derived peptides; Nano-complex formation; Characterization; Stability; SMALL INTERFERING RNA; LOCKED-IN-DENDRIMERS; DENDRITIC CELLS; DELIVERY; OLIGONUCLEOTIDES; DENDRIPLEXES; LIPOSOMES; PROTEIN; SYSTEM; VIRUS;
D O I
10.1016/j.colsurfb.2012.07.011
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Dendrimers have been proposed as new carriers for selected HIV-1 peptides. This paper reports on the complexation behaviour of the three HIV-derived-peptides: Gp160, NH-EIDNYTNTIYTLLEE-COOH; P24, NH-DTINEEAAEW-COOH and Nef, NHGMDDPEREVLEWRFDSRLAF-COOH with second generation cationic carbosilane dendrimers (CBD) branched with carbon-silicon bonds (CBD-CS) or oxygen-silicon bonds (CBD-OS). Studies on the formation of complexes between HIV peptides and CBDs by fluorescence polarization, zeta-potential, electrophoresis and transmission electron microscopy have shown that both studied dendrimers form complexes with HIV peptides. At a molar ratio of (2.5-3):1 (dendrimer:peptide), the complexes formed were in the size range of 180-275 nm and with significant positive surface charge. The results suggest that interactions between dendrimers and HIV peptides have electrostatic nature due to the negative charge of peptides backbone and positive charge of dendrimer functional groups. Dendriplex stability depended on the type of studied dendrimers. Time of peptides release from the complexes ranged from 1 (CBD-OS) to similar to 36 (CBD-CS) h. Basing on the obtained results, we propose that the water-soluble cationic carbosilane dendrimers can be considered for delivery of HIV peptides to dendritic cells. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:236 / 242
页数:7
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