TWO-DIMENSIONAL GEL ELECTROPHORESIS ANALYSIS OF BROWN ALGAL PROTEIN EXTRACTS

被引：39

作者：

Contreras, Loretto ^{[1
]}

Ritter, Andres ^{[1
,2
]}

Dennett, Geraldine ^{[1
]}

Boehmwald, Freddy ^{[3
]}

Guitton, Nathalie ^{[4
]}

Pineau, Charles ^{[4
]}

Moenne, Alejandra ^{[3
]}

Potin, Philippe ^{[2
]}

Correa, Juan A. ^{[1
]}

机构：

[1] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Ctr Adv Studies Ecol & Biodivers, Dept Ecol, Santiago, Chile

[2] Univ Paris 06, CNRS, Biol Stn, UMR 7139, Roscoff, France

[3] Univ Santiago Chile, Dept Biol, Fac Quim & Biol, Santiago, Chile

[4] INSERM, High Throughput Prote Platform OUEST Genopole, UPRES JE 2459, U625, F-35042 Rennes, France

来源：

JOURNAL OF PHYCOLOGY | 2008年 / 44卷 / 05期

关键词：

2-DE; algae; Phaeophyceae; protein extraction methods; proteomics;

D O I：

10.1111/j.1529-8817.2008.00575.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

High-quality protein extracts are required for proteomic studies, a field that is poorly developed for marine macroalgae. A reliable phenol extraction protocol using Scytosiphon gracilis Kogame and Ectocarpus siliculosus (Dillwyn) Lyngb. (Phaeophyceae) as algal models resulted in high-quality protein extracts. The performance of the new protocol was tested against four methods available for vascular plants and a seaweed. The protocol, which includes an initial step to remove salts from the algal tissues, allowed the use of highly resolving two-dimensional gel electrophoresis (2-DE) protein analyses, providing the opportunity to unravel potentially novel physiological processes unique to this group of marine organisms.

引用

页码：1315 / 1321

页数：7

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