Within the context of the support of entrepreneurship development and entrepreneurial thinking and behaviour in the society a large emphasis is presently worldwide placed on motivation of potential, especially graduate, entrepreneurs and preparation of young entrepreneurs by means of integration of entrepreneurship education into the education system. Education institutions play a significant role for business start-ups and should be able to react on actual needs of entrepreneurship education. This paper is focused on problems of entrepreneurial education and linkages between entrepreneurship education and development of entrepreneurship and competitiveness in the Czech Republic. The level of entrepreneurship education and its influence on entrepreneurship development is evaluated thanks to authors' empirical research realized in the year 2010. For this research was used questionnaire method divided in two parts and focused on two respondent groups. Respondents of the first questionnaire were university students with business education. This survey was aimed to relations of university students to entrepreneurship and evaluation of provided school education and its contribution to entrepreneurial skills development. The second questionnaire was carried out by Czech enterprises and was focused on the preparation of the graduates for practice. The aim was to discover the main missing entrepreneurial knowledge and skills. The obtained results were compared with similar existing surveys in the Europe, especially with Eurobarometer. The survey results shown that the level of entrepreneurship education in the Czech Republic ( neither generally in the European Union) is not so positively evaluated. A significant part of interviewing graduates does not feel that provided education influences their interest in entrepreneurship activities. The majority of them prefer a status of an employee than an entrepreneur. They feel to have lack of entrepreneurial competency. Since last years there has been implemented several activities at the Czech universities in order to support graduate entrepreneurs but still it seems not have such extent and system access as at some foreign institutions. There is missing a comprehensive conception of entrepreneurship education and general education system that leads to entrepreneurship behaviour development in the Czech Republic. The entrepreneurship education is provided at some universities on the base of individual access. The survey results demonstrated the insufficient integration of entrepreneurial skills education into education systems in the Czech Republic. Not only the state should react to these facts by creation of entrepreneurship development politics, but especially each university should consider a crucial factors and methods of entrepreneurship education. It must be still considered that investments to the human capital are the decisive factor of competitiveness. Finally, there are stated the recommendation for entrepreneurship education development at the universities in the Czech Republic. Especially, the idea of business incubators aimed to graduate entrepreneurs is discussed.