Assessment of DNA damage and lipid peroxidation in diabetic mice: Effects of propolis and epigallocatechin gallate (EGCG)

被引:61
作者
Orsolic, Nada [1 ]
Sirovina, Damir [1 ]
Gajski, Goran [2 ]
Garaj-Vrhovac, Vera [2 ]
Jembrek, Maja Jazvinscak [3 ]
Kosalec, Ivan [4 ]
机构
[1] Univ Zagreb, Fac Sci, Dept Anim Physiol, Zagreb 10000, Croatia
[2] Inst Med Res & Occupat Hlth, Mutagenesis Unit, Zagreb 10000, Croatia
[3] Rudjer Boskovic Inst, Lab Mol Neuropharmacol, Div Mol Med, Zagreb, Croatia
[4] Univ Zagreb, Dept Microbiol, Fac Pharm & Biochem, Zagreb 10000, Croatia
关键词
Propolis; EGCG; Alloxan; Diabetes; DNA damage; Lipid peroxidation; RADICAL-SCAVENGING ACTIVITY; OXIDATIVE STRESS; CHEMICAL-COMPOSITION; ANTIOXIDANT ACTIVITY; ANTIBACTERIAL ACTIVITY; BIOLOGICAL-PROPERTIES; IDENTIFICATION; ALLOXAN; TEA; QUANTITATION;
D O I
10.1016/j.mrgentox.2013.04.022
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
There is growing recognition that polyphenolic compounds present in many plants and natural products may have beneficial effects on human health. Propolis - a substance produced by honeybees - and catechins in tea, in particular (-)-epigallocatechin gallate (EGCG), are strong antioxidants that appear to have anti-obesity and anti-diabetic effects. The present study was designed to elucidate the anti-diabetic effect of the water-soluble derivative of propolis (WSDP), which contains phenolic acids as the main compounds, and EGCG in alloxan-induced (75 mg/kg, iv) diabetes in mice. Intraperitoneal administration of EGCG or propolis at doses of 50 mg/kg body weight (bw) to diabetic mice for a period of 7 days resulted in a significant increase in body weight and in haematological/immunological blood parameters, as well as in 100% survival of the mice. A significant decrease in lipid peroxidation in liver, kidney and brain tissue was also observed in diabetic mice treated with these two agents. Additionally, EGCG and propolis clearly reduced DNA damage in peripheral lymphocytes of diabetic mice. Our studies demonstrate the anti-oxidative and anti-inflammatory potential of WSDP and EGCG, which could exert beneficial effects against diabetes and the associated consequences of free-radical formation in kidney, liver, spleen and brain tissue. The results suggest that dietary supplementation with WSDP or EGCG could potentially contribute to nutritional strategies for the prevention and treatment of diabetes mellitus. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:36 / 44
页数:9
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